The mechanism analysis of anti-cancer immune escape by tumor suppressor gene REIC

• Project/Area Number: 25861425
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Okayama University
• Principal Investigator: HUANG PENG 岡山大学, 医歯(薬)学総合研究科, 助教 (00610841)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 遺伝子治療 / 癌ワクチン / 骨髄系免疫抑制細胞 / REIC/Dkk3 / REIC/DKK-3 / MDSC / REIC/Dkk-3 / 樹状細胞

Outline of Final Research Achievements

In-situ gene therapy of cancer suppressor gene REIC / Dkk-3 against solid cancer, displayed that the synergistic effects of the "activation of the anti-cancer immunity" and "selective apoptosis of cancer cells", has been demonstrated in several animal models. In this study, REIC / Dkk-3 protein had been elucidated that the suppressive effect on the differentiation of MDSC, furthermore, the mechanism also be verify.

Research Products

• [Journal Article] The cysteine-rich core domain of REIC/Dkk-3 is critical for its effect on monocyte differentiation and tumor regression. (2015)
  • Author(s): Kinoshita R, Watanabe M, Huang P, Li SA, Sakaguchi M, Kumon H, Futami J.
  • Journal Title: Oncology Reports Volume: In press Issue: 6 Pages: 2908-2914
  • DOI: 10.3892/or.2015.3885
  • Peer Reviewed
• [Journal Article] Significant association between the Axin2 rs2240308 single nucleotide polymorphism and the incidence of prostate cancer. (2014)
  • Author(s): Ma C, Liu C, Huang P, Kaku H, Chen J, Guo K, Ueki H, Sakai A, Nasu Y, Kumon H, Shimizu K, Watanabe M.
  • Journal Title: Oncology Letters Volume: 8 Issue: 2 Pages: 789-794
  • DOI: 10.3892/ol.2014.2177
  • Peer Reviewed
• [Journal Article] Dramatic Increase in Expression of a Transgene by Insertion of Promoters Downstream of the Cargo Gene (2014)
  • Author(s): Masakiyo Sakaguchi, Masami Watanabe, Rie Kinoshita, Haruki Kaku, Hideo Ueki, Junichiro Futami, Hitoshi Murata, Yusuke Inoue, Shun-Ai Li, Peng Huang, Endy Widya Putranto, I. Made, Winarsa Ruma, Yasutomo Nasu, Hiromi Kumon, Nam-ho Huh
  • Journal Title: Mol. Biotechnol Volume: 56 Issue: 7 Pages: 621-630
  • DOI: 10.1007/s12033-014-9738-0
  • Peer Reviewed
• [Journal Article] A novel gene expression system strongly enhances the anticancer effects of a REIC/Dkk-3-encoding adenoviral vector (2014)
  • Author(s): Watanabe M., Sakaguchi M., Kinoshita R., Kaku H., Ariyoshi Y., Ueki H., Tanimoto R., Ebara S., Ochiai K., Futami J., Li SA., Huang P., Nasu Y., Huh NH., Kumon H
  • Journal Title: Oncology Reports Volume: 31巻 Issue: 3 Pages: 1089-95
  • DOI: 10.3892/or.2013.2958
  • Peer Reviewed
• [Presentation] Immunomodulation and anti-cancer activity of REIC/Dkk-3 protein
  • Author(s): Peng Huang, Masami Watanabe, Yasutomo Nasu, Haruki Kaku, Hiromi Kumon
  • Organizer: European Society for Gene and Cell Therapy 2013
  • Place of Presentation: Madrid