| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
We made the model animal that had the dysfunction of the spermatogenesis. We investigated stem cell activity in spermatogonial stem cells (SSCs) by using the cryptorchid rat model and stem cell marker “Utf1” (undifferentiated embryonic transcription factor 1). SSCs were the UFT1 positive cell(Active form) and the UFT1 negative cell(Potential form). Next,we investigated the microarray between descended testes and undescended testes. We detected highly the Kdm5a gene. Therefore, we cultured the mouse spermatogonial cell(GC-1) and Kdm5a-Gfp expression vector. We detected lower the H3K4me3 gene by PCR and Western blotting.
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