| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
(OBJECTIVE)DAX1 plays important roles in spermatogenesis in Sertoli cells in the testes. However, DAX1 function in Sertoli cells is unclear. We therefore analyzed the function of DAX1 in Sertoli cells using the adenoviral gene transfer technique.(METHODS) 1) In vitro study: An adenovirus vector was tested on the Sertoli cell line to assess the efficiency of gene transfer. The expression level of Sertoli cell-specific genes was examined by quantitative RT-PCR. 2) In vivo study: Dax1 expression in the testes was analyzed by immunohistochemistry. (RESULTS) 1) Scf was expressed at higher levels after Dax1 gene transfer. 2) Strong DAX1 staining was detected in the nuclei of Sertoli cells.(CONCLUSIONS) Strong Scf expression was observed after Dax1 gene transfer, indicating that the nuclear receptor DAX1 controls SCF expression in Sertoli cells. DAX1 may activate Sertoli cells to stimulate spermatogenesis via a SCF/C-KIT signaling pathway.
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