| Project/Area Number |
25861444
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Nagoya City University |
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Principal Investigator |
Iwatsuki Shoichiro 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (70595397)
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| Research Collaborator |
KUBOTA Hiroki 名古屋市立大学, 大学院医学研究科, 研究員 (10347403)
UMEMOTO Ukihiro 名古屋市立大学, 大学院医学研究科, 講師 (80381812)
SASAKI Shoichi 名古屋市立大学, 大学院医学研究科, 准教授 (50225869)
KOHRI Kenjiro 名古屋市立大学, 学長 (30122047)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 精子形成 / 遺伝子導入 / Notchシグナル / 機能解析 / siRNA / Numb / Numb-like / 精細胞 |
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| Outline of Final Research Achievements |
In this study, we assessed functions of Numb and Numb-like, which are inhibitory factors of Notch signal pathway, in rat spermatogenesis using in vivo gene transfer technique.
By immunohistochemistry, Numb and Numb-like were expressed in spermatocytes and later germ cells; however, Numb expression level was relatively lower in elongated spermatids then in spermatocytes and round spermatids. We constructed vector plasmid expressing siRNAs against Numb or Numb-like, which were transfected to rat testis in vivo. After knocking down of Numb or Numb-like in rat testis resulted in disturbance of differentiation of spermatocytes to spermatids. Our results suggested that Numb and Numb-like regulates differentiation of spermatids by inhibiting Notch signal pathway; Furthermore, it is suggested that Numb and Numb-like acts antagonistically each other.
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