| Project/Area Number |
25861459
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
Tsuiji Kenji 東北大学, 医学(系)研究科(研究院), 助教 (40528155)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 子宮平滑筋肉腫 / クルクミン / モデルマウス / mTOR |
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| Outline of Final Research Achievements |
Uterine leiomyosarcoma generally has an unfavorable response to standard chemotherapy. The active ingredient derived from the herb Curcuma longa, curcumin, shows antitumor properties in a variety of cancer cell lines by altering a number of oncogenetic pathways. To explore the possibility of curcumin as an alternative chemotherapy, we investigated curcumin’s antitumor effect on uterine leiomyosarcoma.
Human leiomyosarcoma cell lines, SKN and SK-UT-1, were cultured for in vitro and in vivo experiments. In vitro study, curcumin significantly reduced SKN and SK-UT-1 cell proliferation. In vivo study, curcumin reduced the volume and mass of uterine leiomyosarcoma significantly with a concordant decrease in mTOR pathway. Cleaved PARP and TUNEL staining increased significantly with curcumin administration, indicating the induction of apoptosis.
In a summary, curcumin inhibited uterine leiomyosarcoma in vitro and in vivo by targeting mTOR pathway and inducing apoptosis.
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