The expression and function of LCN2 in the extravillous trophoblast
Project/Area Number |
25861483
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Obstetrics and gynecology
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Research Institution | Shinshu University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | lipocalin2 / 絨毛外トロフォブラスト / MMP-9活性 / 妊娠初期 / 浸潤能 / 酸素濃度 / E-cadherin / 浸潤能 酸素濃度 |
Outline of Final Research Achievements |
In this study, the function of LCN2 was analyzed using EVTs isolated from early placental tissue and a choriocarcinoma cell line, JAR, as a model of EVT. WST-1 assay revealed that the addition of recombinant LCN2 (rLCN2) did not alter the proliferative activity. Whereas, rLCN2 enhanced the invasive ability (Matrigel invasion assay) and the activity of matrix metalloproteinase-9 (MMP-9) (gelatin zymography) in a dose-dependent manner. Next, we examined the effect of oxygen concentration (21%, 5% and 2%) on LCN2 expression, MMP-9 activity and invasive ability. In both cells, the expression of LCN2, MMP-9 activity and invasive ability were increased in lower O2 level. The invasive ability increased under 2% O2 concentration was markedly inhibited by LCN2-knockdown and restored by rLCN2 addition. These findings suggest that, LCN2 may be involved in regulation of EVT invasiveness in early placentation.
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] Recurrent low-grade endometrial stromal sarcoma with intracaval and intracardiac tumor thrombus: diagnosis, treatment, and surgical management2014
Author(s)
Miyamoto T, Tachibana R, Kobara H, Takano T, Kato H, Shimizu A, Ohya A, Uehara T, Shiozawa T.
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Journal Title
International Cancer Conference Journal
Volume: 3
Issue: 1
Pages: 1-7
DOI
Related Report
Peer Reviewed
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