| Project/Area Number |
25861483
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | lipocalin2 / 絨毛外トロフォブラスト / MMP-9活性 / 妊娠初期 / 浸潤能 / 酸素濃度 / E-cadherin / 浸潤能 酸素濃度 |
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| Outline of Final Research Achievements |
In this study, the function of LCN2 was analyzed using EVTs isolated from early placental tissue and a choriocarcinoma cell line, JAR, as a model of EVT. WST-1 assay revealed that the addition of recombinant LCN2 (rLCN2) did not alter the proliferative activity. Whereas, rLCN2 enhanced the invasive ability (Matrigel invasion assay) and the activity of matrix metalloproteinase-9 (MMP-9) (gelatin zymography) in a dose-dependent manner. Next, we examined the effect of oxygen concentration (21%, 5% and 2%) on LCN2 expression, MMP-9 activity and invasive ability. In both cells, the expression of LCN2, MMP-9 activity and invasive ability were increased in lower O2 level. The invasive ability increased under 2% O2 concentration was markedly inhibited by LCN2-knockdown and restored by rLCN2 addition. These findings suggest that, LCN2 may be involved in regulation of EVT invasiveness in early placentation.
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