Induction of Mn-SOD by cAMP is associated with histone acetylation status aof the C/EBPb binding site of their promoters in human endometrial stromal cells.

• Project/Area Number: 25861495
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Yamaguchi University
• Principal Investigator: TAMURA Isao 山口大学, 医学部附属病院, 助教 (40610663)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 子宮内膜 / 脱落膜化 / エピジェネティクス

Outline of Final Research Achievements

This study showed the new molecular mechanisms by which C/EBPｂ up-regulates the expression of Mn-SOD in human endometrial stromal cell (ESC). The enhancer region of Mn-SOD gene show high histone acetylation status, which may contribute to form the loose chromatin structure so that C/EBPb can easily acces to the DNA binding site. In human ESC, Mn-SOD gene expression is regulated by epigenetic mechanisms.

Research Products

• [Journal Article] Importance ofC/EBPβbinding and histone acetylation status in the promoter regions for induction of IGFBP-1, PRL and Mn-SOD by cAMP in human endometrial stromalcell (2014)
  • Author(s): Isao Tamura, Shun Sato, Maki Okada, Manabu Tanabe, Lifa Lee, Ryo Maekawa, Hiromi Asada, Yoshiaki Yamagata, Hiroshi Tamura, Norihiro Sugino
  • Journal Title: Endocrinology Volume: 155 Pages: 275-286
  • Peer Reviewed
• [Presentation] ヒト子宮内膜間質細胞（ESC）の脱落膜化によって誘導されるmanganese superoxide dismutase (Mn-SOD) 遺伝子発現に関与する新たな転写調節経路 (2013)
  • Author(s): 田村 功、田辺 学、李 理華、前川 亮、浅田裕美、山縣芳明、田村博史、杉野法広
  • Organizer: 日本内分泌学会
  • Place of Presentation: 仙台市(仙台国際センター）
• [Presentation] ヒト子宮内膜間質細胞（ESC）の脱落膜化によって誘導されるmanganese superoxide dismutase (Mn-SOD) 遺伝子発現に関与する新たな転写調節経路 (2013)
  • Author(s): 田村 功、田辺 学、李 理華、前川 亮、浅田裕美、山縣芳明、田村博史、杉野法広
  • Organizer: 日本産婦人科学会学術講演会
  • Place of Presentation: 札幌市（ロイトン札幌）
• [Presentation] Induction of IGFBP-1, PRL and Mn-SOD by cAMP is associated with histone acetylation status of the C/EBPβ binding site of their promoters in human endometrial stromal cells (2013)
  • Author(s): Isao Tamura, Maki Okada, Manabu Tanabe, Lifa Lee, Norihiro Sugino
  • Organizer: Annual meeting of the society for the study on reproduction
  • Place of Presentation: モントリオール/カナダ
• [Presentation] ヒト子宮内膜間質細胞 (ESC) における脱落膜化関連遺伝子発現のエピジェネティクス調節 (2013)
  • Author(s): 田村 功
  • Organizer: 第31回日本受精着床学会総会・学術講演会
  • Place of Presentation: 別府市（別府国際コンベンションセンター）
  • Invited
• [Presentation] 子宮内膜間質細胞（ESC）の脱落膜化によって上昇する遺伝子発現におけるC/EBPβを介したエピジェネティクス調節機構 (2013)
  • Author(s): 田村 功、岡田真紀、李 理華、前川 亮、浅田裕美、佐藤 俊、山縣芳明、田村博史、杉野法広
  • Organizer: 日本エピジェネティクス研究会第７回年会
  • Place of Presentation: 奈良市(奈良県新公会堂）