Genomic factor associated with HPV persistent infection

• Project/Area Number: 25861498
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Nagasaki University
• Principal Investigator: ABE Syuhei 長崎大学, 医歯薬学総合研究科(医学系), 助教 (50549590)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 子宮頸癌 / コピー数多型 / DEFB4 / HPV

Outline of Final Research Achievements

We obtained three heads of results in this study. (1) When considering the cervical cancer prevention and health check system in Japan, to clarify the HPV genotyping of the HPV16 and HPV52 is important. (2) HPV16 and/or 52 genotyping and viral load of cervical scraping cells are useful as a molecular marker to estimate the changes in the cervical cytology. (3) Latest knowledge of an association between cervical cancer and DEFB4 copy-number variation, it was considered useful for the diagnosis and prevention of the onset of cervical cancer.

This study now performs exsome analysis and methylation array analysis using the next-generation sequencing method is expected to be connected to the identification of more host side factors.

Research Products

• [Journal Article] Genome-wide association study of HPV-associated cervical cancer in Japanese women. (2014)
  • Author(s): Miura K, Mishima H, Kinoshita A, Hayashida C, Abe S, Tokunaga K, Masuzaki H, Yoshiura K.
  • Journal Title: J Med Virol. Volume: 86 Pages: 1153-1158
  • Peer Reviewed
• [Journal Article] Single human papillomavirus 16 or 52 infection and later cytological findings in Japanese women with NILM or ASC-US. (2014)
  • Author(s): Abe S, Miura K, Kinoshita A, Mishima H, Miura S, Yamasaki K, Hasegawa Y, Higashijima A, Jo O, Yoshida A, Kaneuchi M, Yoshiura K, Masuzaki H.
  • Journal Title: Journal of Human Genetics Volume: 59 (5) Issue: 5 Pages: 251-255
  • DOI: 10.1038/jhg.2014.9
  • Peer Reviewed
• [Journal Article] Identification of endometrioid endometrial carcinoma-associated microRNAs in tissue and plasma. (2014)
  • Author(s): Tsukamoto O, Miura K, Mishima H, Abe S, Kaneuchi M, Higashijima A, Miura S, Kinoshita A, Yoshiura K, Masuzaki H.
  • Journal Title: Gynecologic Oncology Volume: 132 (3) Issue: 3 Pages: 715-721
  • DOI: 10.1016/j.ygyno.2014.01.029
  • Peer Reviewed
• [Journal Article] Genome-wide association study of HPV-associated cervical cancer in Japanese women. (2014)
  • Author(s): Miura K, Mishima H, Kinoshita A, Hayashida C, Abe S, Tokunaga K, Masuzaki H, Yoshiura KI.
  • Journal Title: Journal of Medical Virology Volume: -
  • Peer Reviewed
• [Journal Article] Single human papillomavirus 16 or 52 infection and later cytological findings in Japanese women with NILM or ASC-US. (2014)
  • Author(s): Abe S, Miura K, Kinoshita A, Mishima H, Miura S, Yamasaki K, Hasegawa Y, Higashijima A, Jo O, Yoshida A, Kaneuchi M, Yoshiura KI, Masuzaki H.
  • Journal Title: Journal of Human Genetics Volume: -
  • Peer Reviewed
• [Journal Article] Identification of endometrioid endometrial carcinoma-associated microRNAs in tissue and plasma. (2014)
  • Author(s): Tsukamoto O, Miura K, Mishima H, Abe S, Kaneuchi M, Higashijima A, Miura S, Kinoshita A, Yoshiura K, Masuzaki H.
  • Journal Title: Gynecologic Oncology Volume: 132 Pages: 715-721
  • Peer Reviewed
• [Journal Article] Predominantly placenta-expressed mRNAs in maternal plasma as predictive markers for twin-twin transfusion syndrome. (2014)
  • Author(s): Miura K, Higashijima A, Miura S, Mishima H, Yamasaki K, Abe S, Hasegawa Y, Kaneuchi M, Yoshida A, Kinoshita A, Yoshiura K, Masuzaki H.
  • Journal Title: Prenatal Diagnosis Volume: 34 Pages: 345-349
  • Peer Reviewed
• [Journal Article] Initial viral load in cases of single human papillomavirus 16 or 52 persistent infection is associated with progression of later cytopathological findings in the uterine cervix. (2013)
  • Author(s): Hamaguchi D, Miura K, Abe S, Kinoshita A, Miura S, Yamasaki K, Yoshiura K, Masuzaki H.
  • Journal Title: Journal of Medical Virology Volume: 85 Pages: 2093-2100
  • Peer Reviewed
• [Journal Article] Copy number variation of the antimicrobial-gene, defensin beta 4, is associated with susceptibility to cervical cancer. (2013)
  • Author(s): Abe S, Miura K, Kinoshita A, Mishima H, Miura S, Yamasaki K, Hasegawa Y, Higashijima A, Jo O, Sasaki K, Yoshida A, Yoshiura K, Masuzaki H.
  • Journal Title: Journal of Human Genetics Volume: 58 Pages: 250-253
  • Peer Reviewed
• [Journal Article] Characterization of placenta-specific microRNAs in fetal growth restriction pregnancy. (2013)
  • Author(s): Higashijima A, Miura K, Mishima H, Kinoshita A, Jo O, Abe S, Hasegawa Y, Miura S, Yamasaki K, Yoshida A, Yoshiura K, Masuzaki H.
  • Journal Title: Prenatal Diagnosis Volume: 33 Pages: 214-222
  • Peer Reviewed
• [Presentation] 発癌性ヒトパピローマウイルス感染と細胞診所見（NILMあるいはASC-US）の推移との関連 (2014)
  • Author(s): 阿部修平、三浦清徳、金内優典、増崎英明
  • Organizer: 日本婦人科腫瘍学会
  • Place of Presentation: 栃木県総合文化センター（栃木県）
  • Year and Date: 2014-07-17 – 2014-07-19
• [Presentation] ヒトパピローマウィルス16型および 52型感染細胞の2年後の所見 (2013)
  • Author(s): 阿部修平、三浦清徳、東島愛、河野通晴、山崎健太郎、三浦生子、小寺宏平、藤下晃、鮫島哲郎、布施正樹、村上誠、池本理恵、増崎英明
  • Organizer: 第54回日本婦人科腫瘍学会学術講演会
  • Place of Presentation: 東京都港区台場２－６－１ホテルグランパシフィック LE DAIBA
• [Remarks] 長崎大学産婦人科ホームページ
  • URL: http://www.med.nagasaki-u.ac.jp/gyneclgy/