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Methylation analysis by using digital PCR in head and neck cancer

Research Project

Project/Area Number 25861546
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Otorhinolaryngology
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

Endo Shiori  浜松医科大学, 医学部附属病院, 医員 (10625205)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
KeywordsDigital PCR法 / メチル化解析 / 頭頸部癌 / Digital PCR / Digital PCR装置
Outline of Final Research Achievements

Bisulfite-treated DNA was amplified by PCR with two designed pairs of methylation-specific PCR primers for the promoter region of the SALL3 gene. Two
million PCR reactions using conventional TaqMan assays with a 96-well plate workflow. Three applications demonstrate that the massive partitioning afforded by our ddPCR system provides orders of magnitude more precision and sensitivity than real-time PCR. ddPCR system will allow researchers to explore complex genetic landscapes, discover and validate new disease associations, and define a new era of molecular diagnostics.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (9 results)

All 2016 2015 2014 2013

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Acknowledgement Compliant: 3 results,  Open Access: 2 results) Presentation (4 results)

  • [Journal Article] Misawa K, Misawa Y, Kanazawa T, Mochizuki D, Imai A, Endo S, Carey TE, Mineta H2016

    • Author(s)
      Misawa K, Misawa Y, Kanazawa T, Mochizuki D, Imai A, Endo S, Carey TE, Mineta H
    • Journal Title

      Clin Exp Metastasis.

      Volume: 32 Issue: 2 Pages: 187-195

    • DOI

      10.1007/s10585-015-9768-4

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Primary combined small and squamous cell carcinoma of the hypopharynx: A case report.2016

    • Author(s)
      Kiyoshi Misawa Hideya Kawasaki Shiori Endo Daiki Mochizuki Kotaro Morita Yuichi Hashimoto Yuki Misawa Hirotoshi Kikuchi Takeharu Kanazawa Toshihide Iwashita Hiroyuki Mineta
    • Journal Title

      Molecular and Clinical Oncology

      Volume: 4(5) Issue: 5 Pages: 709-714

    • DOI

      10.3892/mco.2016.788

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Tumor suppressor activity and inactivation of galanin receptor type 2 by aberrant promoter methylation in head and neck cancer2014

    • Author(s)
      Misawa Y, Misawa K, Kanazawa T, Uehara T, Endo S, Mochizuki D, Yamatodani T, Carey TE, Mineta H
    • Journal Title

      Cancer

      Volume: 120 Issue: 2 Pages: 205-213

    • DOI

      10.1002/cncr.28411

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prognostic value of Type XXII and XXIV collagen mRNA expression in head and neck cancer patients2014

    • Author(s)
      "Misawa K, Kanazawa T, Imai A, Endo S, Mochizuki D, Fukushima H, Misawa Y, Mineta H
    • Journal Title

      Molecular and Clinical Oncology

      Volume: 2 Pages: 285-291

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Frequent promoter hypermethylation of tachykinin-1 and tachykinin receptor type 1 is a potential biomarker for head and neck cancer.2013

    • Author(s)
      Misawa K*, Kanazawa T, Misawa Y, Imai A, Uehara T, Mochizuki D, Endo S, Takahashi G, Mineta H
    • Journal Title

      J Cancer Res Clin

      Volume: 100 Issue: 5 Pages: 879-889

    • DOI

      10.1007/s00432-013-1393-5

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] 切開排膿術を要した乳幼児頸部膿瘍の7症例2015

    • Author(s)
      遠藤志織
    • Organizer
      第76回耳鼻咽喉科臨床学会
    • Place of Presentation
      浜松
    • Year and Date
      2015-06-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] 頭頸部癌におけるneuropeputid FF受容体遺伝子の発現とプロモーター領域のメチル化との関連について2015

    • Author(s)
      三澤 清、今井篤志、望月大極、遠藤志織、近藤玄樹、三澤由幾、金澤丈治、峯田周幸
    • Organizer
      第116回日本耳鼻咽喉科学会総会
    • Place of Presentation
      東京
    • Year and Date
      2015-05-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] 頭頸部癌におけるオレキシン遺伝子の発現とプロモーター領域のメチル化との関連について2014

    • Author(s)
      三澤 清、今井篤志、遠藤志織、望月大極、三澤由幾、高橋吾郎、金澤丈治、峯田周幸
    • Organizer
      第115回日本耳鼻咽喉科学会総会
    • Place of Presentation
      福岡
    • Year and Date
      2014-05-21 – 2014-05-23
    • Related Report
      2014 Research-status Report
  • [Presentation] 頭頸部癌におけるソマトスタチン遺伝子の発現とプロモーター領域のメチル化との関連について2013

    • Author(s)
      三澤 清、金澤丈治、今井篤志、三澤由幾、上原貴行、遠藤志織、望月大極、杉山健一、高橋吾郎、峯田周幸
    • Organizer
      第114回日本耳鼻咽喉科学会総会
    • Place of Presentation
      札幌
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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