Methylation analysis by using digital PCR in head and neck cancer
| Project/Area Number |
25861546
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
Endo Shiori 浜松医科大学, 医学部附属病院, 医員 (10625205)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Digital PCR法 / メチル化解析 / 頭頸部癌 / Digital PCR / Digital PCR装置 |
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| Outline of Final Research Achievements |
Bisulfite-treated DNA was amplified by PCR with two designed pairs of methylation-specific PCR primers for the promoter region of the SALL3 gene. Two
million PCR reactions using conventional TaqMan assays with a 96-well plate workflow. Three applications demonstrate that the massive partitioning afforded by our ddPCR system provides orders of magnitude more precision and sensitivity than real-time PCR. ddPCR system will allow researchers to explore complex genetic landscapes, discover and validate new disease associations, and define a new era of molecular diagnostics.
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Report
(4 results)
Research Products
(9 results)
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[Journal Article] Misawa K, Misawa Y, Kanazawa T, Mochizuki D, Imai A, Endo S, Carey TE, Mineta H2016
Author(s)
Misawa K, Misawa Y, Kanazawa T, Mochizuki D, Imai A, Endo S, Carey TE, Mineta H
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Journal Title
Clin Exp Metastasis.
Volume: 32
Issue: 2
Pages: 187-195
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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