Functional Analysis of c-Cbl Interacting Protein of 85 kDa in Head and Neck Squamous Cell Carcinoma.

• Project/Area Number: 25861567
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: National Hospital Organization, Kyushu Cancer Center (2014-2015); Kyushu University (2013)
• Principal Investigator: Wakasaki Takahiro 独立行政法人国立病院機構(九州がんセンター臨床研究センター), その他部局等, その他 (10608871)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: CIN85 / Head and Neck / Squamous Cell Carcinoma / 頭頸部扁平上皮癌 / 頭頸部癌 / EGFR

Outline of Final Research Achievements

In this study, it was suggested that CIN85 went through the PI3K/Akt pathway as well as Ras/MAPK pathway, which contributes to a tumor progress in head and neck squamous cell carcinoma (HNSCC).
I showed that CIN85 right related to elevating invasion ability other than increasing proliferative ability in the HNSCC cell line in this study. However, I was not able to prove a meaningful relationship between CIN85 and EMT-related genes. According to both gene expression analyses and western blotting analyses of HNSCC cell lines (control v. s. CIN85 knock down), it is elucidated the participation of the PI3K / Akt pathway. CIN85 is an adapter gene, and the joint molecule diverges into many branches. CIN85 may contribute to the phenotype of cancer by various pathways.