New elucidation of the oxytocin system involvement in head and neck cancer pain relief using a fluorescent gene

• Project/Area Number: 25861602
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Kyushu University (2014); National Hospital Organization, Kyushu Medical Center (Clinical Institute) (2013)
• Principal Investigator: KATO AKIKO 九州大学, 大学病院, その他 (90412650)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 遺伝子改変動物 / オキシトシン / 蛍光タンパク遺伝子 / 口内炎 / 蛍光タンパク / 癌性疼痛

Outline of Final Research Achievements

Stomatitis model was created by contacting the acetic acid-containing filter paper in the oral mucosa of oxytocin-mRFP1 transgenic rats, and the activation of oxytocin neurons localized in the hypothalamic supraoptic and paraventricular nuclei (PVN) was quantified by mRFP1 red fluorescent intensity as changes of the expression of the oxytocin gene. In stomatitis model group, mRFP1 red fluorescent intensity in the parvocellular and magnocellular divisions of the PVN has been increased significantly in comparison with control group. This result suggests that stomatitis-induced nociceptive stimulation may cause activation of oxytocin neurons in the PVN. Further study should examine whether hypothalamic oxytocinergic pathways may be involved in regulation of pain in head and neck during inflammation and cancer.

Research Products

• [Journal Article] Florescentvisualisation of the hypothalamic oxytocin neurones activated by cholecystokinin-8 in rats expressing c-fos-enhanced green fluorescent protein and oxytocin-monomeric redfluorescent protein 1 fusion transgenes. (2014)
  • Author(s): Katoh, A. Shoguchi, K. Matsuoka, H. Yoshimura, M. Ohkubo, JI. Matsuuta, T. Maruyama, T. Ishikura, T. Atitomi, T. Fujihara, H. Hashimoto, H. Suzuki, H. Murphy, D. & Ueta, Y.
  • Journal Title: Journal of Neuroendocrinology Volume: 26 Pages: 341-347
  • Peer Reviewed
• [Journal Article] A c-fos-monomeric red fluorescent protein l fusion transgene is differentially expressed in rat forebrain and brainstem after chronic dehydration and rehydration (2013)
  • Author(s): Yoshimura, M. Ohkubo, J. Katoh, A. Ohno, M. Ishikura, T. Kakuma, T. Yoshimatsu, H. Murphy, D. Ueta, Y
  • Journal Title: Journal of Neuroendocrinology Volume: 25(5) Issue: 5 Pages: 478-487
  • DOI: 10.1111/jne.12022
  • Peer Reviewed
• [Journal Article] 伝子改変技術を用いたオキシトシンニューロンの蛍光タンパクによる可視化の試み (2013)
  • Author(s): 加藤 明子、上田 陽一、鈴木 秀明
  • Journal Title: J UOEH（産業医科大学雑誌） Volume: 35 Pages: 165-171
  • Peer Reviewed
• [Presentation] The Oxytocin-monomeric red fluorescent protein 1 fusion gene expressing after central administration of colchicine in transgenic rat. (2014)
  • Author(s): Hashimoto, H. Matsuura, T. Yoshimura, Ohkubo, Katoh, A. Maruyama, T. & Ueta, Y.
  • Organizer: 第37回内藤カンファレンス
  • Place of Presentation: 北海道ニセコ町
  • Year and Date: 2014-07-15 – 2014-07-18
• [Presentation] 口内炎モデルにおける視床下部オキシトシン発現の変動～オキシトシン-mRFP1ラットを用いた検討～ (2014)
  • Author(s): 加藤 明子、松浦 孝紀、吉村 充弘、丸山 崇、橋本 弘史、上田 陽一
  • Organizer: 第36回日本疼痛学会
  • Place of Presentation: KKRホテル大阪
  • Year and Date: 2014-06-20 – 2014-06-21
• [Presentation] ラット大細胞性神経分泌ニューロンにおけるオキシトシン分泌顆粒と神経活動の蛍光タンパクによる可視化 (2014)
  • Author(s): 加藤 明子、松浦 孝紀、大久保 淳一、吉村 充弘、丸山 崇、橋本 弘史、上田 陽一
  • Organizer: 第87回日本内分泌学会学術総会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] 下垂体後葉ホルモン研究における蛍光タンパクの応用：トランスジェニックラットによる浸透圧負荷の検討 (2013)
  • Author(s): 佐藤慧太、越智拓海、モリス ジョン F 、加藤明子、上田陽一、坂本竜哉、坂本浩隆
  • Organizer: 第12回九州脳・高血圧・循環制御研究会
  • Place of Presentation: 徳島
• [Presentation] 下垂体後葉ホルモン研究における蛍光タンパクの応用：トランスジェニックラットによる浸透圧負荷の検討
  • Author(s): 加藤明子、松浦孝紀、大久保淳一、吉村充弘、丸山 崇、橋本弘史、上田陽一
  • Organizer: 第12回九州脳・高血圧・循環制御研究会
  • Place of Presentation: 福岡