The development of a new therapy in diabetic retinopathy by application of alpha-glucosylhesperidin

• Project/Area Number: 25861609
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Asahikawa Medical College
• Principal Investigator: Yokota Harumasa 旭川医科大学, 医学部, 助教 (60431417)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 糖尿病網膜症 / 虚血性網膜症 / 神経保護 / 血管保護 / 分散ヘスペレチン / 糖転移ヘスペリジン

Outline of Final Research Achievements

The present study for the first time demonstrated that water-dispersible hesperetin (WD-Hpt) decreased the number of cell death in the ganglion cell layer after ischemia reperfusion (I/R) injury in the retina. This beneficial protective effect of WD-Hpt on the retina derived from reduction of oxidative stress. Consequently, this led to alleviation of activated microglia and reduction of chronic inflammation. Therefore, WD-Hpt is likely to protect neural cells in the retina from diabetes by reducing oxidative stress and chronic inflammation. Moreover, WD-Hpt was shown to reduce I/R-induced gliosis in Muller cells. This also indicates that WD-Hpt can be used for the treatment in diabetic macular edema.

Research Products

• [Journal Article] Neuroprotective effect of water-dispersible hesperetin in retinal ischemia reperfusion injury (2016)
  • Author(s): Shimouchi A, Yokota H, Ono S, Matsumoto C, Tamai T, Takumi H, Narayanan SP, Kimura S, Kobayashi H, Caldwell RB, Nagaoka T, Yoshida A
  • Journal Title: Japanese Journal of Ophthalmology Volume: 60 Issue: 1 Pages: 51-61
  • DOI: 10.1007/s10384-015-0415-z
  • NAID: 210000170569
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Serum prorenin levels are not associated with ocular diseases in non-diabetic subjects (2015)
  • Author(s): Yokota H
  • Journal Title: J Renin Angiotensin Aldosterone Syst Volume: 16 Issue: 1 Pages: 153-158
  • DOI: 10.1016/j.ajo.2015.04.021
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] The role of prorenin in microglial activation (2015)
  • Author(s): Yokota H
  • Organizer: The Association for Research in Vision and Ophthalmology
  • Place of Presentation: Denver in the USA
  • Year and Date: 2015-05-03 – 2015-05-08
• [Presentation] The role of renin-angiotensin system in the regulation of retinal arterioles (2014)
  • Author(s): Harumasa Yokota
  • Organizer: The Association for Research in Vision and Ophthalmology
  • Place of Presentation: Orland, USA
• [Presentation] 分散ヘスペレチンは虚血性網膜症の神経節細胞死を抑制する (2013)
  • Author(s): 下内昭人、横田陽匡
  • Organizer: 第１１７回日本眼科学会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] Water-dispersible hesperetin prevents ganglion cell loss in the retina after retinal ischemia reperfusion injury (2013)
  • Author(s): Akihito Shimouchi, Harumasa Yokota
  • Organizer: The Association for Research in Vision and Ophthalmology
  • Place of Presentation: Seattle, USA