Analysis of spatial distribution of synapses and morphology of the retinal ganglion cells.

Research Project

Project/Area Number	25861612
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Ophthalmology
Research Institution	Tohoku University
Principal Investigator	MORITOH Satoru 東北大学, 医学(系)研究科(研究院), 助手 (20647234)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	網膜神経節細胞 / 遺伝子銃 / 網膜培養 / シナプス / 緑内障
Outline of Final Research Achievements	I developed organotypic tissue culture of porcine retina with particle-mediated gene transfer. Using this method, I successfully transfected cell morphological marker plasmid and synaptic marker plasmid into the porcine retinal ganglion cells. I examined morphology and synaptic position of the porcine retinal ganglion cells. I found several types of bistratified retinal ganglion cells including a candidate for direction-selective ganglion cells in porcine retinas.