| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
Similar to other neurodegenerative diseases, the incidence of glaucoma increases with aging. We used in vivo (living mice) and in vitro imaging of the retinal ganglion cells (RGCs) to reveal the disturbances of axonal transport in glaucoma models and age-related changes of axonal transport.
Importantly, we have, for the first time, succeeded in imaging of axonal transport of mitochondria in the mammalian central nervous system in vivo. This in vivo imaging of mouse RGCs with a two-photon microscope showed that the number of transported mitochondria decreased before RGC death. In contrast, with increasing age up to 23-25 months, axonal transport of mitochondria (distance, duration, and duty cycle) shortened. Also, in vitro imaging showed the disturbances of axonal transport after laser-induced axonal injury. In vivo imaging of axonal transport in RGCs may lead to the prediction of RGC death in glaucoma patients.
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