| Project/Area Number |
25861635
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Ehime University |
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Principal Investigator |
OKA Naoko 愛媛大学, 医学部附属病院, 医員 (20403818)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | アカントアメーバ / 光線力学療法 / PDT / メチレンブルー / 角膜感染症 / 真菌 / 光感受性物質 |
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| Outline of Final Research Achievements |
We sought to elucidate the efficacy of the photodynamic therapy (PDT) against Acanthamoeba keratitis using methylene blue (MB) which is a photosensitizer. In vitro, Antiacanthamoeba activity was evaluated by checking the respiratory activity of the amoeba. MB-PDT suppressed the respiratory activity of trophozoites in an MB-concentration-dependent manner. Also anti-amoebic effect of MB-PDT was observed to be light dose dependent. Besides, we examined the toxicity of PDT treatment in vivo using C57BL/6 mouse corneas with epithelial defects, and found that MB-PDT did not cause any appreciable damage to the cornea. Thus, we conclude that PDT treatment would produce minimal toxicity in vivo. Although our results show that MB-PDT is efficacious in vitro, these results may not correlate with in vivo efficacy; therefore, further animal studies are under way to test the in vivo efficacy of this treatment for Acanthamoeba.
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