Development of high quality corneal epithelial cell sheet
| Project/Area Number |
25861639
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 眼科 / 角膜 / 経上皮電気抵抗値 / 再生医療 / 薬物毒性 / 角膜上皮細胞シート移植 / タイトジャンクション / 角膜上皮 / バリア機能 |
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| Outline of Final Research Achievements |
In this study, the quality of the cultivated corneal epithelial cell sheets used for treatment of severe corneal diseases was evaluated by the corneal transepithelial electrical resistance (TER). TER increased after construction of tight junctions among the corneal epithelial cells. The corneal epithelial cell sheets with high TER value were successfully transplanted to the rabbit cornea. It is confirmed that evaluation of TER have a potential to prognosticate the results of corneal epithelial cell sheet transplantation clinically.
Furthermore, the factors that would affect the quality of the corneal epithelial cells are examined by TER study. Especially benzalkonium chloride in eye drops used after corneal transplantation damaged the corneal epithelium. However additives such as polyoxyethylene castor oil have a protective effect on corneal damage by benzalkonium chloride.
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Report
(3 results)
Research Products
(2 results)
