| Project/Area Number |
25861699
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Research Collaborator |
KURITA Erina 杏林大学, 医学部, 専攻医
SHIOTA Noriko 杏林大学, 医学部, 実験助手
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 単球・マクロファージ系細胞 / 線維芽細胞 / 創傷治癒 / 単球 / マクロファージ / 線維化 |
|---|
| Outline of Final Research Achievements |
The effects of monocyte/macrophage lineage cells on proliferation and gene expression of fibroblasts were examined in the co-culture system. Monocyte/macrophage lineage cells were purified after passaged culture of mononuclear cells from peripheral blood using temperature-responsive culture dish. Co-culture with monocyte/macrophage lineage cells enhanced proliferation of fibroblasts. Under co-culture, expression of type 1 collagen and transforming growth factor beta 1 was down-regulated in fibroblasts, while expression of matrix metalloproteinase 1 was up-regulated. Similar results were obtained when culture supernatant of monocyte/macrophage lineage cells was added to fibroblasts. It was suggested that, in the process of wound healing, monocyte/macrophage lineage cells enhance proliferation of fibroblasts and have anti-fibrotic effects on fibroblasts via humoral factors.
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