Interaction between mnonocyte/macrophage lineage cells and fibroblasts in the process of wound healing

Research Project

Project/Area Number	25861699
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Plastic surgery
Research Institution	Kyorin University
Principal Investigator	SUGA Hirotaka 杏林大学, 医学部, 助教 (60633972)
Research Collaborator	KURITA Erina 杏林大学, 医学部, 専攻医 SHIOTA Noriko 杏林大学, 医学部, 実験助手
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords	単球・マクロファージ系細胞 / 線維芽細胞 / 創傷治癒 / 単球 / マクロファージ / 線維化
Outline of Final Research Achievements	The effects of monocyte/macrophage lineage cells on proliferation and gene expression of fibroblasts were examined in the co-culture system. Monocyte/macrophage lineage cells were purified after passaged culture of mononuclear cells from peripheral blood using temperature-responsive culture dish. Co-culture with monocyte/macrophage lineage cells enhanced proliferation of fibroblasts. Under co-culture, expression of type 1 collagen and transforming growth factor beta 1 was down-regulated in fibroblasts, while expression of matrix metalloproteinase 1 was up-regulated. Similar results were obtained when culture supernatant of monocyte/macrophage lineage cells was added to fibroblasts. It was suggested that, in the process of wound healing, monocyte/macrophage lineage cells enhance proliferation of fibroblasts and have anti-fibrotic effects on fibroblasts via humoral factors.