Histological Study of Costal Cartilage after Transplantation and Reasons for Avoidance of Postoperative Absorption and Retain Cartilage Structure in Animal model
Project/Area Number |
25861712
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Plastic surgery
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Research Institution | Kurume University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 軟骨移植 / 軟骨膜除去 / GFP transgenic rat / FIB/SEM tomography / 間葉系幹細胞 / 自家移植モデル / 自家組織移植 / 微細構造解析 / 軟骨膜 / 線維芽細胞 / 軟骨 / 組織移植 / 形成外科 / 三次元構造解析 / 経時的変化 / 3次元構造解析 |
Outline of Final Research Achievements |
The sections of costal cartilage was harvested from 10-week-old wild rats and transplanted to dorsal region of 10-week-old green fluorescent protein (GFP) transgenic rat. These were subcutaneously harvested from GFP rats at 4-weekes and 8- weeks. This experiment was carried out in 2 randomized groups, with the perichondrium removed from the transplanted cartilage in one group, and with intact perichondrium transplanted. The GFP-positive chondrocytes in 8 weeks later in the group of the perichondrium removed. The GFP-positive chondrocytes was not replaced in the group of perichondrium intact. This result showed cartilage was reproduced from membrane-like tissue regenerated around cartilage after removing the perichondrium; continuously generating new chondrocytes.
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Report
(5 results)
Research Products
(3 results)