The expression of NETs in bronchial aspirate of patient with ARDS

• Project/Area Number: 25861718
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Osaka University
• Principal Investigator: HIROSE Tomoya 大阪大学, 医学(系)研究科(研究院), 特任助教(常勤) (70597509)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: NETs / ARDS / 好中球 / 集中治療 / 呼吸不全 / 集中治療医学 / NETｓ / ステロイド / 感染

Outline of Final Research Achievements

NETs are fibrous structures that are released extracellularly from activated neutrophils in response to infection. NETs rapidly trap and kill pathogens as a first-line defense for infection. On the other hands, it was reported that the overexpression of NETs had harmful effects to tissue. The purpose of this study is to evaluate whether NETs are produced in bronchial aspirate of patient with ARDS.
In this study, NETs formation was observed in bronchial aspirates of all of the patients diagnosed as ARDS. In survivors, the expression of NETs was observed and the P/F ratio improved gradually. After that, NETs were fragmented. On the other hands, in non-survivors, the expression of NETs was observed too, but the expression of NETs persisted. NETs formation seems to be a predictive factor of prognosis of these conditions.

Research Products

• [Journal Article] Presence of neutrophil extracellular traps and citrullinated histone h3 in the bloodstream of critically ill patients (2014)
  • Author(s): Hirose T, Hamaguchi S, Matsumoto N, et a
  • Journal Title: PLoS One Volume: Nov 13;9(11) Issue: 11 Pages: e111755-e111755
  • DOI: 10.1371/journal.pone.0111755
  • NAID: 120006986309
  • Peer Reviewed / Open Access
• [Journal Article] 重症病態におけるNETs(Neutrophil Extracellular Traps) (2013)
  • Author(s): 廣瀬 智也，濱口 重人，松本 直也，竹川 良介、山本 倫久、小島 将裕，入澤 太郎，関 雅文，田崎 修，山本 紘司，小倉 裕司，朝野 和典，嶋津 岳士
  • Journal Title: 日本Shock学会雑誌 Volume: 28 Pages: 55-60
• [Presentation] The presence of neutrophil extracellular traps in bronchial aspirate of patient diagnosed as ARDS or acute exacerbation of idiopathic pulmonary fibrosis (2015)
  • Author(s): Ojima M, Yamamoto N, Hirose T, et al.
  • Organizer: 第 35 回ISICEM (International Symposium on Intensive Care and Emergency Medicine)
  • Place of Presentation: ベルギー ブリュッセル
  • Year and Date: 2015-03-17 – 2015-03-20
• [Presentation] ARDS患者における喀痰中のNeutrophil Extracellular Traps (NETs)形成と臨床経過の検討 (2014)
  • Author(s): 小島将裕、山本倫久、廣瀬智也、他
  • Organizer: 第42回日本救急医学会総会・学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2014-10-28 – 2014-10-30
• [Presentation] Neutrophil Extracellular Traps under critical condition (2014)
  • Author(s): Hamaguchi Shigeto, Hirose Tomoya, Masafumi Seki, Naoya Matsumoto, Yukihiro Akeda, Norihisa Yamamoto, Osamu Tasaki, Takeshi Shimazu, Kazunori Tomono
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島
• [Presentation] 重症病態におけるNETs(Neutrophil Extracellular Traps) (2013)
  • Author(s): 廣瀬 智也, 濱口 重人, 松本 直也, 竹川 良介, 山本 倫久, 入澤 太郎, 関 雅文, 田崎 修, 山本 紘司, 朝野 和典, 嶋津 岳士
  • Organizer: 第28回日本Shock学会総会
  • Place of Presentation: 東京
• [Presentation] 重症患者における血液中のNETs (Neutrophil Extracellular Traps)とシトルリン化ヒストンH3 (2013)
  • Author(s): 廣瀬智也、濱口重人、松本直也、入澤太郎、竹川良介、山本倫久、関雅文、田崎修、山本紘司、朝野和典、嶋津岳士
  • Organizer: 第41回 日本救急医学会総会・学術集会
  • Place of Presentation: 東京