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Matrix metalloproteinases-9 and VEGF in edema after traumatic brain injury in mice

Research Project

Project/Area Number 25861719
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Emergency medicine
Research InstitutionOsaka University

Principal Investigator

HOSOMI Sanae  大阪大学, 医学(系)研究科(研究院), 特任研究員 (90644005)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords脳浮腫 / 頭部外傷 / 炎症
Outline of Final Research Achievements

Many basic and clinical studies suggest the strong relationship between MMP-9 and brain edema. However, detailed information regarding MMP-9 secretion after blunt TBI is still unavailable. In this study, we analyzed what type of immune cells expresses MMP-9 in the injured brain using CCI model mice. MMP-9 is secretor enzyme, and both microglia and macrophages have same antigen, so traditional immunohistochemistry analysis can’t quantify what type of immune cells secret MMP-9. That is why we used flow cytometry intracellular analysis. MMP-9 positive cells are detected in the injured brain over 7days, but not sham mice. And these MMP-9 positive cells are positive both CD11b+/Gr-1hi. We confirmed these cell sorted by FACS inhibited T cell proliferation in vitro. In conclusion, cells expressed MMP-9 after TBI are myeloid-derived suppressor cells accumulated in the injured site but not resident immune cells in the brain.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2015 2014

All Presentation (10 results) (of which Invited: 1 results)

  • [Presentation] 頭部外傷後の慢性神経炎症の新たな診断ツール:TSPO-PETの応用2015

    • Author(s)
      細見早苗、藤田幸、渡部直史、小山佳久、山下俊英、小倉裕司、嶋津岳士
    • Organizer
      第42回日本集中治療医学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2015-02-09 – 2015-02-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 頭部外傷後の神経炎症イメージング:18F-DPA714-PETによる評価2015

    • Author(s)
      細見早苗
    • Organizer
      第3回臨床免疫核医学研究会
    • Place of Presentation
      大阪
    • Year and Date
      2015-01-19
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 頭部外傷後の神経炎症イメージング:18F-DPA714-PET2014

    • Author(s)
      細見早苗、渡部直史、金井泰和、池田隼人、小山佳久、山下俊英、畑澤 順、小倉裕司、嶋津岳士
    • Organizer
      第54回日本核医学会学術総会
    • Place of Presentation
      大阪
    • Year and Date
      2014-11-06 – 2014-11-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] 18kDa translocator protein(TSPO)PET による頭部外傷後の神経炎症イメージング2014

    • Author(s)
      細見早苗、渡部直史、山下俊英、畑澤 順、小倉裕司、嶋津岳士
    • Organizer
      第42回日本救急医学会総会・学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2014-10-28 – 2014-10-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] TSPO-PET imaging after traumatic brain injury in mice2014

    • Author(s)
      Sanae Hosomi, Kazuhisa Yoshiya, Tadashi Watabe, Toshihide Yamashita, Hiroshi Ogura, Takeshi Shimazu
    • Organizer
      The 32nd Annual Symposium of the National Naurotrauma Society
    • Place of Presentation
      アメリカ、サンフランシスコ
    • Year and Date
      2014-06-29 – 2014-07-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] Why Matrix metalloproteinases (MMP)-9 concentration in blood reflects the fragility after traumatic brain injury?2014

    • Author(s)
      Sanae Hosomi, Toshihide Yamashita, Hiroshi Ogura, Takeshi Shimazu
    • Organizer
      International conference on Emergency Medicine
    • Place of Presentation
      中国、香港
    • Year and Date
      2014-06-11 – 2014-06-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Matrix metalloproteinases-9 secretion from accumulated Myeloid-derived suppressor cells after traumatic brain injury in mice2014

    • Author(s)
      Sanae Hosomi, Toshihide Yamashita, Hiroshi Ogura, Takeshi Shimazu
    • Organizer
      The 43rd Critical Care Congress
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2013 Research-status Report
  • [Presentation] マウス脳挫傷モデルにおけるmatrix metalloproteinase (MMP)-9産生細胞の動態解明2014

    • Author(s)
      細見早苗、山下俊英、小倉裕司、嶋津岳士
    • Organizer
      第41回日本集中治療医学会学術集会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report
  • [Presentation] Infiltrating Myeloid-derived suppressor cells (MDSCs) secret Matrix metalloproteinases-9 after traumatic brain injury in mice2014

    • Author(s)
      Sanae Hosomi, Toshihide Yamashita, Hiroshi Ogura, Takeshi Shimazu
    • Organizer
      International Neurotrauma Society 2014
    • Place of Presentation
      Budapest, Hungary
    • Related Report
      2013 Research-status Report
  • [Presentation] マウス脳挫傷モデルにおけるMyeloid-derived suppressor cells (MDSC)の発現とmatrix metalloproteinase(MMP)-9分泌2014

    • Author(s)
      細見早苗、山下俊英、小倉裕司、嶋津岳士
    • Organizer
      第114回日本外科学会定期学術集会
    • Place of Presentation
      京都
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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