| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Many basic and clinical studies suggest the strong relationship between MMP-9 and brain edema. However, detailed information regarding MMP-9 secretion after blunt TBI is still unavailable. In this study, we analyzed what type of immune cells expresses MMP-9 in the injured brain using CCI model mice. MMP-9 is secretor enzyme, and both microglia and macrophages have same antigen, so traditional immunohistochemistry analysis can’t quantify what type of immune cells secret MMP-9. That is why we used flow cytometry intracellular analysis. MMP-9 positive cells are detected in the injured brain over 7days, but not sham mice. And these MMP-9 positive cells are positive both CD11b+/Gr-1hi. We confirmed these cell sorted by FACS inhibited T cell proliferation in vitro. In conclusion, cells expressed MMP-9 after TBI are myeloid-derived suppressor cells accumulated in the injured site but not resident immune cells in the brain.
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