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The role of TAF12 in bone marrow stromal cells on osteoclastogenesis

Research Project

Project/Area Number 25861745
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Morphological basic dentistry
Research InstitutionThe University of Tokushima

Principal Investigator

TERAMACHI Jumpei  徳島大学, ヘルスバイオサイエンス研究部, 助教 (20515986)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords骨髄ストローマ細胞 / TAF12 / 活性化ビタミンD3 / RANKL / 破骨細胞 / 活性型ビタミンD3
Outline of Final Research Achievements

We previously found that transcription initiation factor TFIID subunit 12 (TAF12), a coactivator of the vitamin D receptor is increased in the osteoclast precursors or bone marrow stromal cells. However the role of increased TAF12 on osteoclastogenesis is unclear. Therefore, we aimed to clarify the role of TAF12 in bone marrow stromal cell support of osteoclast formation and function. TNF-α and IL-6 upregulates the expression of TAF12. Increased TAF12 expression induces hyper-sensitivity to 1,25-(OH)2D3. RANKL expression and osteoclast formation were induced at very low levels of 1,25-(OH)2D3. In immunoprecipitation assay, TAF12 and ATF7 physically interact in stromal cells. Knockdown of TAF12 in IL-6-induced TAF12 expressing stromal cell decreased hyper-sensitivity to 1,25-(OH)2D3; as well as RANKL expression and osteoclast formation. These results show that TAF12 contributes to the hypersensitivity of bone marrow stromal cells to 1,25-(OH)2D3 in inflammatory bone disease.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (29 results)

All 2015 2014 2013

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (22 results) (of which Invited: 2 results)

  • [Journal Article] Pim-2 kinase is an important target of treatment for tumor progression and bone loss in myeloma.2015

    • Author(s)
      Hiasa M, Teramachi J, Oda A, Amachi R, Harada T, Nakamura S, Miki H, Fujii S, Kagawa K, Watanabe K, Endo I, Kuroda Y, Yoneda T, Tsuji D et al.
    • Journal Title

      Leukemia

      Volume: 29 Issue: 1 Pages: 207-217

    • DOI

      10.1038/leu.2014.147

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Double stranded RNA-dependent protein kinase is necessary for TNF-α-induced osteoclast formation in vitro and in vivo.2015

    • Author(s)
      Shinohara H, Teramachi J, Okamura H, Yang D, Nagata T, Haneji T.
    • Journal Title

      Journal of Cellular Biochemistry

      Volume: 116 Issue: 9 Pages: 877-888

    • DOI

      10.1002/jcb.25151

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Increased IL-6 expression in osteoclasts is necessary but not sufficient for the development of Paget's disease of bone.2014

    • Author(s)
      Teramachi J, Zhou H, Subler MA, Kitagawa Y, Galson DL, Dempster DW, Windle JJ, Kurihara N, Roodman GD.
    • Journal Title

      Journal of Bone and Mineral Research.

      Volume: 29 Issue: 6 Pages: 1456-1465

    • DOI

      10.1002/jbmr.2158

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduction of PP2A Cα stimulates adipogenesis by regulating the Wnt/GSK-3β/β-catenin pathway and PPARγ expression2014

    • Author(s)
      Okamura H, Yang D, Teramachi J, Yoshida K, Haneji T
    • Journal Title

      BBA-Molecular Cell Research

      Volume: 1843 Issue: 11 Pages: 2376-2384

    • DOI

      10.1016/j.bbamcr.2014.06.008

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Calcium hydroxide suppresses the virulence of lipopolysaccharide from Porphyromonas endodontalis to bone cells2014

    • Author(s)
      Guo J, Yang D, Okamura H, Teramachi J, Ochiai K, Qiu L, Haneji T
    • Journal Title

      Journal of Dental Research

      Volume: 93 Issue: 5 Pages: 508-513

    • DOI

      10.1177/0022034514526886

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Role of ATF7-TAF12 interactions in the vitamin D response hypersensitivity of osteoclast precursors in Paget's disease.2013

    • Author(s)
      Teramachi J, Hiruma Y, Ishizuka S, Ishizuka H, Brown JP, Michou L, Cao H, Galson DL, Subler MA, Zhou H, Dempster DW, Windle JJ, Roodman GD, Kurihara N.
    • Journal Title

      Journal of Bone and Mineral Research

      Volume: 28 Issue: 6 Pages: 1489-1500

    • DOI

      10.1002/jbmr.1884

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Okadaic acid activates PKR pathway and induces apoptosis through PKR-stimulation in osteoblastic MG63 cells2013

    • Author(s)
      Haneji T, Hirashima K, Teramachi J, Morimoto H
    • Journal Title

      International Journal of Oncology

      Volume: (in press) Issue: 6 Pages: 1904-10

    • DOI

      10.3892/ijo.2013.1911

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] Critical role of PKR in TNF-α-induced osteoclastogenesis2015

    • Author(s)
      Teramachi J, Yang D, Okamura H, Morimoto H, Haneji T
    • Organizer
      第120回日本解剖学会総会・全国学術集会
    • Place of Presentation
      神戸国際会議場(兵庫県・神戸市)
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Pim2を標的とした骨髄腫骨病変の新規治療法の開発2014

    • Author(s)
      寺町順平
    • Organizer
      第17回癌と骨病変研究会
    • Place of Presentation
      千代田放送会館(東京都・千代田区)
    • Year and Date
      2014-11-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Therapeutic impact of Pim inhibition on myeloma bone disease: blockade of NF-κB-mediated suppression of osteoblastogenesis and stimulation of osteoclastogenesis2014

    • Author(s)
      Teramachi J, Hiasa M, Oda A, Harada T, Amachi R, Kagawa K, Miki H, Nakamura S, Fujii S, Endo I, Matsumoto T, Abe M.
    • Organizer
      第76回日本血液学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府・大阪市)
    • Year and Date
      2014-10-31 – 2014-11-02
    • Related Report
      2014 Annual Research Report
  • [Presentation] プロテインホスファターゼPP2A C alphaは,脂肪細胞分化に関与する2014

    • Author(s)
      岡村 裕彦、楊 諦、羽地 達次、寺町 順平
    • Organizer
      日本解剖学会第69回中国・四国支部学術集会
    • Place of Presentation
      広島大学 広仁会館(広島県・広島市)
    • Year and Date
      2014-10-25 – 2014-10-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] PKRによる炎症性骨破壊制御2014

    • Author(s)
      寺町順平、篠原宏貴、稲垣裕司、楊諦、岡村裕彦、永田俊彦、羽地達次
    • Organizer
      日本解剖学会第69回中国・四国支部学術集会
    • Place of Presentation
      広島大学 広仁会館(広島県・広島市)
    • Year and Date
      2014-10-25 – 2014-10-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] PKRは歯周病におけるLPSおよびTNF-αによる破骨細胞形成促進の重要な因子である2014

    • Author(s)
      寺町順平、森本景之、岡村裕彦、羽地達次
    • Organizer
      第56回歯科基礎医学会
    • Place of Presentation
      福岡国際会議場(福岡県・福岡市)
    • Year and Date
      2014-09-26 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 脂肪細胞分化におけるプロテインホスファターゼPP2A Cα の役割2014

    • Author(s)
      岡村 裕彦、寺町 順平、羽地 達次
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡国際会議場(福岡県・福岡市)
    • Year and Date
      2014-09-26 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Critical Role of Pim-2 in NF-kB-mediated Suppression of Osteoblastogenesis and Stimulation of Osteoclastogenesis: Therapeutic Impact of Pim Inhibition on Myeloma Bone Disease.2014

    • Author(s)
      Teramachi J, Hiasa M, Oda A, Amachi R, Harada T, Nakamura S, Kagawa K, Miki H, Fujii S, Watanabe K, Endo I, Matsumoto T, Abe M.
    • Organizer
      2014 American Society for Bone Mineral Researc Annual Meeting
    • Place of Presentation
      George R. Brown Convention Center (ヒューストン・アメリカ合衆国)
    • Year and Date
      2014-09-12 – 2014-09-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] Increased Expression of TAF12 in the Bone Microenvironment in Multiple Myeloma Enhances Tumor Cell Growth and Osteoclast Formation2014

    • Author(s)
      Kitagawa Y, Teramachi J, Windle JJ, Chirgwin JM, Roodman GD, Kurihara N.
    • Organizer
      2014 American Society for Bone Mineral Researc Annual Meeting
    • Place of Presentation
      George R. Brown Convention Center(ヒューストン・アメリカ合衆国)
    • Year and Date
      2014-09-12 – 2014-09-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] MVNP Expression in Osteoclasts Induces IGF1 to Increase EphrinB2/EphB4 and Osteoblast Differentiation2014

    • Author(s)
      Teramachi J, Kitagawa Y, Windle JJ, Michou L, Brown JP, Kurihara N, Roodman GD.
    • Organizer
      2014 American Society for Bone Mineral Researc Annual Meeting
    • Place of Presentation
      George R. Brown Convention Center(ヒューストン・アメリカ合衆国)
    • Year and Date
      2014-09-12 – 2014-09-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] A Novel Sequestosome-1 / p622 ZZ Domain Inhibitor Blocks TNFα Induced Suppression of OBL Differentiation in MM2014

    • Author(s)
      Silbermann R, Teramachi J, Mohammad K, Zhao W, Zhou D, Yang P, Eiseman JL, Xie XQ, Roodman GD, Kurihara N
    • Organizer
      2014 American Society for Bone Mineral Researc Annual Meeting
    • Place of Presentation
      George R. Brown Convention Center(ヒューストン・アメリカ合衆国)
    • Year and Date
      2014-09-12 – 2014-09-15
    • Related Report
      2014 Annual Research Report
  • [Presentation] PKRは歯周病変における破骨細胞形成及び骨吸収を制御する。2014

    • Author(s)
      寺町順平、稲垣裕司、岡村裕彦、永田俊彦、羽地達次
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府・大阪市)
    • Year and Date
      2014-07-24 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] 骨形成・骨芽細胞分化におけるプロテインホスファターゼPP2A Cαの役割2014

    • Author(s)
      岡村 裕彦、 寺町 順平、 羽地 達次
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府・大阪市)
    • Year and Date
      2014-07-24 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Pim-2キナーゼはTNF-αによる骨芽細胞分化抑制および破骨細胞形成促 進の必須媒介因子である:Pim阻害薬の骨髄腫骨病変改善効果2014

    • Author(s)
      寺町順平、日浅雅博、小田明日香、天知良太、中村信元、遠藤逸朗、松本俊夫、安倍正博
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場(大阪府・大阪市)
    • Year and Date
      2014-07-24 – 2014-07-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] PKR阻害によるTNF-α誘導性骨吸収の抑制2014

    • Author(s)
      篠原宏貴、寺町順平、稲垣裕司、木戸淳一、羽地達次、永田俊彦
    • Organizer
      第140回日本歯科保存学会春季学会学術大会
    • Place of Presentation
      びわ湖ホール(滋賀県・大津市)
    • Year and Date
      2014-06-19 – 2014-06-20
    • Related Report
      2014 Annual Research Report
  • [Presentation] TNF-α誘導性破骨細胞形成におけるPKRの役割2014

    • Author(s)
      篠原 宏貴、寺町 順平、稲垣 裕司、木戸 淳一、永田 俊彦、羽地 達次
    • Organizer
      第57回春季歯周病学会学術大会
    • Place of Presentation
      長良川国際会議場(岐阜県・岐阜市)
    • Year and Date
      2014-05-23 – 2014-05-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] 歯周病微小環境での破骨細胞形成におけるPKRの役割2014

    • Author(s)
      寺町順平、篠原宏貴、稲垣裕司、森本景之、永田俊彦、羽地達次
    • Organizer
      第119回日本解剖学会総会・全国学術集会
    • Place of Presentation
      自治医科大学(栃木県)
    • Related Report
      2013 Research-status Report
  • [Presentation] IGF1 Contributes to the Increased Bone Formation Induced by Measles Virus Nucleocapsid Protein Expressed by Osteoclasts in Paget’s Bone Disease2013

    • Author(s)
      1. Kurihara N, Teramachi J, Kitagawa Y, Windle JJ, Roodman GD.
    • Organizer
      Annual Meeting of American Society for Bone and Mineral Research
    • Place of Presentation
      Baltimore, MD. USA
    • Related Report
      2013 Research-status Report
  • [Presentation] Increased Expression of TAF12 in Myeloma Cells and the Bone Microenvironment Enhances Tumor Cell Growth and Osteoclast Formation2013

    • Author(s)
      2. Kurihara N, Teramachi J, Kitagawa Y, Chirgwin JM, Roodman GD.
    • Organizer
      Annual Meeting of American Society for Bone and Mineral Research
    • Place of Presentation
      Baltimore, MD. USA
    • Related Report
      2013 Research-status Report
  • [Presentation] PKRは炎症性骨破壊において重要な役割を果たしている2013

    • Author(s)
      寺町順平
    • Organizer
      第54回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      航空会館(東京都)
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] PKRは炎症性骨破壊において重要な役割を果たしている2013

    • Author(s)
      寺町順平、森本景之、羽地達次
    • Organizer
      第55回歯科基礎医学会
    • Place of Presentation
      岡山コンベンションセンター(岡山県)
    • Related Report
      2013 Research-status Report
  • [Presentation] 歯周病変における破骨細胞形成にPKRが関与する2013

    • Author(s)
      寺町順平、平島寛司、羽地達次
    • Organizer
      第32回分子病理学研究会
    • Place of Presentation
      竹林院群芳園(奈良県)
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2023-03-16  

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