Investigation for the pathogenic potential of streptolysin S homolog secreted from beta-hemolytic oral streptococci

• Project/Area Number: 25861746
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: The University of Tokushima
• Principal Investigator: TABATA Atsushi 徳島大学, ソシオテクノサイエンス研究部, 助教 (10432767)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ストレプトリジンS / 口腔連鎖球菌 / アンギノーサス群連鎖球菌 / β溶血 / 病原因子 / Streptococcus

Outline of Final Research Achievements

In this study, we revealed that a homolog of streptolysin S (SLS), a typical peptide-type hemolytic factor secreted from Pyogenic group streptococci, was the β-hemolytic factor of β-hemolytic Anginosus group streptococci (β-AGS). Moreover, the investigation of cytotoxicity of β-hemolytic S. anginosus subsp. anginosus (β-SAA) dependent on the SLS homolog showed that relatively milder than hemolysis but distinguishable cell damage was induced in human oral squamous cell carcinoma under the co-culture condition with β-SAA. These results suggest the participation of SLS homolog secreted from β-AGS not only in β-hemolysis but also in cytotoxicity of β-AGS.

Research Products

• [Journal Article] Molecular analysis of Streptococcus anginosus-derived SagA peptides (2014)
  • Author(s): Yuki Kawaguchi, Atsushi Tabata, Hideaki Nagamune, Kazuto Ohkura
  • Journal Title: Anticancer Research Volume: 34 Pages: 4627-4631
  • Peer Reviewed
• [Journal Article] A streptolysin S homologue is essential for β-haemolytic Streptococcus constellatus subsp. constellatus cytotoxicity (2014)
  • Author(s): Atsushi Tabata, Yuji Sato, Kentaro Maya, Kota Nakano, Ken Kikuchi, Robert Whiley, Kazuto Ohkura, Toshifumi Tomoyasu, Hideaki Nagamune
  • Journal Title: Microbiology Volume: 160 Issue: 5 Pages: 980-991
  • DOI: 10.1099/mic.0.075580-0
  • Peer Reviewed
• [Presentation] S. anginosus subsp. whileyiおよびS. constellatus subsp. viborgensisが保有するβ溶血因子の特性解析 (2015)
  • Author(s): 眞屋健太朗，田端厚之，大倉一人，菊池賢，友安俊文，長宗秀明
  • Organizer: 第88回日本細菌学会総会
  • Place of Presentation: 長良川国際会議場（岐阜県岐阜市）
  • Year and Date: 2015-03-26 – 2015-03-28
• [Presentation] Streptolysin S Homologues of Peptide Hemolysin: Novel Beta-Hemolytic Factors Distributed among Beta-Hemolytic Anginosus Group Streptococci (2014)
  • Author(s): Atsushi Tabata, Kota Nakano, Yuji Sato, Kentaro Maya, Hiromi Ohtani, Yukimasa Ohkubo, Kazuto Ohkura, Ken Kikuchi, Robert A. Whiley, Toshifumi Tomoyasu, Hideaki Nagamune
  • Organizer: XIX Lancefield International Symposium on Streptococci and Streptococcal Diseases
  • Place of Presentation: Buenos Aires（Argentina）
  • Year and Date: 2014-11-09 – 2014-11-12
• [Presentation] β溶血性アンギノーサス群レンサ球菌が保有するストレプトリジンSホモログの多様性 (2014)
  • Author(s): 田端厚之、眞屋健太朗、大谷浩美、佐藤裕士、中野晃太、大倉一人、菊池賢、友安俊文、長宗秀明
  • Organizer: 第46回レンサ球菌研究会
  • Place of Presentation: 東京大学農学部弥生キャンパス（東京都文京区）
  • Year and Date: 2014-06-27 – 2014-06-28
• [Presentation] β溶血性Streptococcus anginosusが保有するストレプトリジンSホモログの特徴と細胞障害性への寄与
  • Author(s): 田端厚之、中野晃太、大倉一人、菊池賢、友安俊文、長宗秀明
  • Organizer: 第22回Lancefieldレンサ球菌研究会
  • Place of Presentation: ホテル 島根イン青山（東京都）