Evaluation of novel antibacterial agents derived from oral cavity against Staphylococcus aureus to inhibit oral colonization.

• Project/Area Number: 25861749
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Kagoshima University
• Principal Investigator: KAWADA-MATSUO Miki 鹿児島大学, 医歯(薬)学総合研究科, 講師 (20527048)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 黄色ブドウ球菌 / 口腔内定着 / バクテリオシン / 二成分制御系 / 抗菌性因子 / 口腔内定着阻害

Outline of Final Research Achievements

We evaluated a novel antibacterial agents against Staphylococcus aureus to inhibit oral colonization. We isolated Streptococcus sanguinis and Streptococcus parasanguinis which have antibacterial actuvuty against S. aureus. These bacteria produce hydrogen peroxide as antibacterial agent. Furthermore, two-component systems of S.aureus are revealed that related to the resistant system against hydrogen peroxide of S. sanguinis and bacteriocins produced by L. lactis and S. warneri. We consider that TCSs can be applied as a fixing inhibitors preventing oral colonization of S. aureus.

Research Products

• [Journal Article] Involvement of the novel two-component NsrRS and LcrRS systems in distinct resistance pathways against nisin A and nukacin ISK-1 in Streptococcus mutans (2013)
  • Author(s): Miki Kawada-Matsuo, Yuichi Oogai, Takeshi Zendo, Jun-ichi Nagao, Yukie Shibata, Yoshihisa Yamashita, Yoshitoshi Ogura, Tetsuya Hayashi, Kenji Sonomoto & Hitoshi Komatsuzawa
  • Journal Title: Appl. Eniviron. Microbiol Volume: 79 Issue: 15 Pages: 4751-4755
  • DOI: 10.1128/aem.00780-13
  • Peer Reviewed
• [Journal Article] Three distinct two-component systems are involved in resistance to the class I bacteriocins, Nukacin ISK-1 and nisin A, in S ta phy lococcus aureus (2013)
  • Author(s): Miki Kawada-Matsuo, Y uuma Y oshida, T akeshi Zendo, Jun-ichi Nagao, Y uichi Oogai, Kenji Sonomoto, Y asunori Nakamura, Norifumi Nakamura & Hitoshi Komatsuzawa
  • Journal Title: PLoS One Volume: 8(7) Issue: 7 Pages: e69455-e69455
  • DOI: 10.1371/journal.pone.0069455
  • Peer Reviewed
• [Presentation] ETB産生黄色ブドウ球菌が産生するバクテリオシンに対する自己耐性機構の解明 (2015)
  • Author(s): 松尾 美樹、加藤 紀文、菅井 基行、小松澤 均
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 岐阜市長良川国際会議場
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] 黄色ブドウ球菌の3組の二成分制御系因子によるclass Iバクテリオシン耐性機構 (2014)
  • Author(s): 松尾美樹、小松澤均
  • Organizer: 歯科基礎医学会総会
  • Place of Presentation: 福岡市福岡国際会議場
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 病原性細菌であるメチシリン耐性黄色ブドウ球菌 MRSA の口腔内定着を阻害する口腔内細菌の探索 (2014)
  • Author(s): 泉 彩夏、松尾 美樹、小松澤 均
  • Organizer: 歯科基礎医学会総会
  • Place of Presentation: 福岡市福岡国際会議場
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] 齲蝕細菌の二成分制御系を介した生存戦略機構 (2014)
  • Author(s): 松尾美樹、小松澤均
  • Organizer: 第87回日本細菌学会総会
  • Place of Presentation: 東京
  • Invited
• [Remarks] 鹿児島大学 医歯学総合研究科 口腔細菌学分野HP
  • URL: http://www.hal.kagoshima-u.ac.jp/dental/Saikin/