Characterization of heme acquisition system of periodontal bacteria

• Project/Area Number: 25861750
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Showa University
• Principal Investigator: Fukamachi Haruka 昭和大学, 歯学部, 助教 (10433799)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 歯周病原細菌 / P. intermedia / 鉄獲得機構 / Prevotella intermedia

Outline of Final Research Achievements

Prevotella intermedia, an oral bacterium associated with periodontal disease, requires heme for growth. Since P. intermedia does not have the complete heme biosynthetic pathway, this pathogen must have some means of acquiring the iron from red blood cells it needs to survive in its relevant host. Heme acquisition from red blood cells by P. intermedia usually involves hemolysis, hemoglobin binding, and the degradation of hemoglobin with the subsequent release of heme. In order to clarify the involvement of heme acquisition system, we firstly have investigated the ability of haemoglobin degradation by P. intermedia cysteine proteases. Next, to investigate the effect of iron availability on the expression of the cysteine protease genes and heme acquisition pathway genes, real-time RT-PCR analysis was performed on cDNA prepared from RNA extracted from P. intermedia grown to mid-exponential phase under iron-repleted and iron-depleted conditions.

Research Products

• [Journal Article] Determination of reactive oxygen generated from natural medicines and their antibacterial activity (2016)
  • Author(s): Tajima N, Takasaki M, Fukamachi H, Igarashi T, Nakajima Y, Arakawa H.
  • Journal Title: Journal of Pharmaceutical Analysis Volume: in press Issue: 4 Pages: 214-218
  • DOI: 10.1016/j.jpha.2016.04.003
  • Peer Reviewed
• [Journal Article] Effects of Hangeshashinto on Growth of Oral Microorganisms. (2015)
  • Author(s): Fukamachi H, Matsumoto C, Omiya Y, Arimoto T, Morisaki H, Kataoka H, Kadena M, Funatsu T, Fukutake M, Kase Y, Kuwata H.
  • Journal Title: Evidence-Based Complementary and Alternative Medicine Volume: 2015 Pages: 512947-512947
  • DOI: 10.1155/2015/512947
  • Peer Reviewed / Open Access
• [Journal Article] Antibacterial Activities of Persimmon Extracts Relate with Their Hydrogen Peroxide Concentration (2014)
  • Author(s): Arakawa H, Takasaki M, Tajima N, Fukamachi H, Igarashi T.
  • Journal Title: Biological and Pharmaceutical Bulletin Volume: 37 Issue: 7 Pages: 1119-1123
  • DOI: 10.1248/bpb.b13-00952
  • NAID: 130004057433
  • ISSN: 0918-6158, 1347-5215
  • Peer Reviewed / Open Access
• [Journal Article] Rothia dentocariosa induces TNF-alpha production in a TLR2-dependent manner. (2014)
  • Author(s): Kataoka H, Taniguchi M, Fukamachi H, Arimoto T, Morisaki H, Kuwata H.
  • Journal Title: Pathog Dis. Volume: 71(1) Issue: 1 Pages: 65-8
  • DOI: 10.1111/2049-632x.12115
  • Peer Reviewed / Open Access
• [Journal Article] Identification and functional analysis of the gene cluster for fructan utilization in (2013)
  • Author(s): Haruka Fuse, Haruka Fukamachi, Mitsuko Inoue, Takeshi Igarashi
  • Journal Title: Prevotella intermediaGene Volume: 515 Issue: 2 Pages: 291-297
  • DOI: 10.1016/j.gene.2012.12.023
  • Peer Reviewed
• [Presentation] Prevotella intermedia extracellular nucleases contribute to degradation of neutrophil extracellular traps (2014)
  • Author(s): Haruka Fukamachi, Midori Doke, Hirobumi Morisaki, Takafumi Arimoto, Hideo Kataoka, and Hirotaka Kuwata
  • Organizer: Japanese Association for Dental Research
  • Place of Presentation: 大阪市
  • Year and Date: 2014-12-04 – 2014-12-05