| Project/Area Number |
25861750
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 歯周病原細菌 / P. intermedia / 鉄獲得機構 / Prevotella intermedia |
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| Outline of Final Research Achievements |
Prevotella intermedia, an oral bacterium associated with periodontal disease, requires heme for growth. Since P. intermedia does not have the complete heme biosynthetic pathway, this pathogen must have some means of acquiring the iron from red blood cells it needs to survive in its relevant host. Heme acquisition from red blood cells by P. intermedia usually involves hemolysis, hemoglobin binding, and the degradation of hemoglobin with the subsequent release of heme. In order to clarify the involvement of heme acquisition system, we firstly have investigated the ability of haemoglobin degradation by P. intermedia cysteine proteases. Next, to investigate the effect of iron availability on the expression of the cysteine protease genes and heme acquisition pathway genes, real-time RT-PCR analysis was performed on cDNA prepared from RNA extracted from P. intermedia grown to mid-exponential phase under iron-repleted and iron-depleted conditions.
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