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Novel strategy for enrichment and isolation of osteoprogenitor cells from iPS cells and the effectiveness in vivo

Research Project

Project/Area Number 25861761
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional basic dentistry
Research InstitutionTokyo Dental College

Principal Investigator

SHINO HIROMI  東京歯科大学, 歯学部, 助教 (00445446)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsiPS細胞 / 骨芽細胞 / 骨細胞 / 骨芽細胞分化 / 骨分化 / iPS cells
Outline of Final Research Achievements

In this study, we developed a new method to stimulate osteogenic differentiation in tissue-nonspecific alkaline phosphatase (TNAP)-positive cells liberated from human induced pluripotent stem cells (hiPSCs)-derived embryoid bodies (EBs) with 14 days long. TNAP is a marker protein of osteolineage cells. We analyzed and isolated TNAP-positive by fluorescence-activated cell sorting. Treating hiPSCs-derived cells with a combination of FGF-2, IGF-1, and TGF-β generated TNAP-positive cells at high frequency. The isolated cells expressed high levels of osterix, which is an exclusive osteogenic marker. Culturing these TNAP-positive cells in osteoblast differentiation medium (OBM) led to the expression of various osteogenic markers. Furthermore, in OBM they were capable of generating many mineralized nodules with strong expression of receptor activator of NF-kappaB ligand and sclerostin (SOST). Real-time RT-PCR showed a significant increase in the expression of osteocyte marker genes.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2013

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (5 results)

  • [Journal Article] Akt Activation is Required for TGF-β1-Induced Osteoblast Differentiation of MC3T3-E1 Pre-Osteoblasts.2015

    • Author(s)
      Suzuki E, Ochiai-Shino H, Aoki H, Onodera S, Saito A, Saito A, Azuma T
    • Journal Title

      Plosons

      Volume: 9(12) Issue: 12 Pages: e112566-e112566

    • DOI

      10.1371/journal.pone.0112566

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Promoting effect of 1,25(OH)2 vitamin D3 in osteogenic differentiation from induced pluripotent stem cells to osteocyte like cells2015

    • Author(s)
      Kato H, Ochiai-Shino H, Onodera S, Saito A, Takahiko Shibahara, Azuma T
    • Journal Title

      Open Biology 5(2):140201

      Volume: 5(2) Issue: 2 Pages: 140201-140201

    • DOI

      10.1098/rsob.140201

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A novel strategy for enrichment and isolation of osteoprogenitor cells from induced pluripotent stem cells based on surface marker combination.2014

    • Author(s)
      Ochiai-Shino H, Kato H, Sawada T, Onodera S, Saito A, Takato T, Shibahara T, Muramatsu T, Azuma T.
    • Journal Title

      PLOS ONE

      Volume: 9 Issue: 6 Pages: e99534-e99534

    • DOI

      10.1371/journal.pone.0099534

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] iPS細胞から骨前駆細胞分化誘導および単離の新規方法開発と骨細胞様細胞への分化誘導2014

    • Author(s)
      東 俊文, 齋藤 暁子, 小野寺 晶子, 加藤 宏, 篠 宏美
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] iPS細胞から誘導した骨芽細胞を用いた創薬評価 活性型ビタミンD3の効果判定2014

    • Author(s)
      加藤 宏, 篠 宏美, 小野寺 晶子, 齋藤 暁子, 林 宰央, 長谷川 大悟, 柴原 孝彦, 東 俊文
    • Organizer
      第32回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] iPS細胞から誘導した骨芽細胞を用いた創薬評価2014

    • Author(s)
      加藤 宏, 篠 宏美, 小野寺 晶子, 齋藤 暁子, 林 宰央, 長谷川 大悟, 柴原 孝彦, 東 俊文
    • Organizer
      第68回NPO法人日本口腔科学会学術集会
    • Place of Presentation
      京王プラザホテル
    • Year and Date
      2014-05-08 – 2014-05-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒトiPS細胞の骨芽細胞への分化誘導時におけるALP発現の動態についての検討2014

    • Author(s)
      齋藤 暁子、篠 宏美、小野寺 晶子、東 俊文
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒトiPS細胞から骨細胞への効果的な誘導2013

    • Author(s)
      篠 宏美、加藤 宏、齋藤 暁子、小野寺 晶子、佐藤 裕、東 俊文
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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