Identification of the responsibility gene on 7p, 8q and 17q about cervical lymph node metastases of oral squamous cell carcinoma.
| Project/Area Number |
25861773
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Pathobiological dentistry/Dental radiology
|
|---|
| Research Institution | Oita University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 口腔扁平上皮癌 / 頚部リンパ節転移 / 頸部リンパ節転移 / 網羅的発現解析 / Ｒｅａｌ Ｔｉｍｅ ＰＣＲ法 / 免疫組織化学法 / アレイＣＧＨ解析 / siＲＮＡ / レンチウイルスシステム / Real Time PCR法 / アレイCGH解析 |
|---|
| Outline of Final Research Achievements |
I identified chromosome aberration domain of 7ｐ12, 8ｑ24 and 17ｑ25 by array comparative genomic hybridization analysis about cervical lymph node metastases of oral squamous cell carcinoma. I performed exhaustive gene expression analysis about 16 oral squamous cell carcinoma cell line, and extracted the gene to develop with the genome copy number amplification of the chromosome domain of 7ｐ12, 8ｑ24 and 17ｑ25. I analyzed candidate gene by the Real Time PCR assay and immunohistochemical analysis. I make the candidate gene expression virus by using a lentiviral system, subsequently, I perform a functional analysis experiment.
|
Report
(3 results)
Research Products
(2 results)
