| Project/Area Number |
25861780
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | 薬物性歯肉増殖症 / 歯肉線維芽細胞 / 18α-グリチルレチン酸 / 細胞増殖 / アポトーシス / 細胞周期 / 薬物誘発性歯肉増殖症 / フェニトイン / 培養ヒト歯肉線維芽細胞 / カスパーゼ / アムロジピン / 歯肉由来線維芽細胞 |
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| Outline of Final Research Achievements |
This investigation aimed to establish the basis of a pharmacotherapy for drug-induced gingival overgrowth. Gingival overgrowth has been attributed to the enhanced growth of gingival fibroblasts. In this study, we investigated the effects of 18-alpha-glycyrrhetinic acid (GA) on growth, the cell cycle, and apoptosis and on the regulators of these processes in gingival fibroblasts isolated from the patients. GA significantly decreased cell numbers and significantly increased the percentage of cells in the sub-G1 and G0/G1 phases of the cell cycle and the number of apoptotic cells. Nuclear condensation and fragmentation of the cells into small apoptotic bodies appeared in the fibroblasts treated with GA. GA significantly decreased the protein levels of cyclins A and D1, CDKs 2 and 6, phosphorylated Rb (ser780 and ser807/811), Bcl-xL and Bcl-2 and increased the protein levels of p27, cytosolic cytochrome c, pro-caspase-3, and cleaved caspase-3 and the activities of caspases 3 and 9.
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