Breakthrough of fluoride-functional mechanism and establishment of its utilization for oral Actinomyces

• Project/Area Number: 25861783
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Tohoku University
• Principal Investigator: Kawashima Junko 東北大学, 東北メディカル・メガバンク機構, 助教 (50633707)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: Actinomyces / Streptococcus / 根面齲蝕 / 糖代謝 / 重炭酸 / 歯学 / 増殖 / 酸産生 / 窒素源 / Fluoride

Outline of Final Research Achievements

Both Streptococcus and Actinomyces can produce acids from dietary sugars and are frequently found in caries lesions. In addition to glycolysis (EMP pathway), Actinomyces species have a pathway that utilizes part of the tricarboxylic acid cycle to combine PEP (a metabolic intermediate of the EMP pathway) with bicarbonate to produce succinate. This study aimed to elucidate the effects of nitrogenous compounds on acid production. Tryptone enhanced glucose-derived acid production. Addition of glutamate increased glucose-derived acid production. These results suggest that nitrogenous compounds in the oral cavity promote acid production by Streptococcus and Actinomyces in vivo.

Research Products

• [Journal Article] Nitrogenous compounds stimulate glucose-derived acid production by oral Streptococcus and Actinomyces (2015)
  • Author(s): Yuka Norimatsu,Junko Kawashima, Teruko Takano-Yamamoto, Nobuhiro Takahashi
  • Journal Title: Microbiology and Immunology Volume: 59 Issue: 9 Pages: 501-506
  • DOI: 10.1111/1348-0421.12283
  • NAID: 40020601160
  • Peer Reviewed
• [Journal Article] Fluoride-sensitivity of growth and acid production of oral Actinomyces : comparison with oral Streptococcus (2013)
  • Author(s): Kawashima J, Nakajo K, Washio J, Mayanagi G, Shimauchi H, Takahashi N
  • Journal Title: Microbiol Immunol Volume: 57(12) Issue: 12 Pages: 797-804
  • DOI: 10.1111/1348-0421.12098
  • Peer Reviewed
• [Presentation] Lactose and glucose metabolism of oral Bifidobacterium and its fluoride-inhibition. (2017)
  • Author(s): Manome A, Abiko Y, Kawashima J, Fukumoto S, Takahashi N
  • Organizer: The 95h IADR General Session & Exhibition
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2017-03-23
• [Presentation] 口腔Bifidobacterium属のグルコースおよびラクトース代謝とフッ化物による抑制効果 (2017)
  • Author(s): 馬目歩実、安彦友希、川嶋順子、福本敏、高橋信博
  • Organizer: 第６回 口腔保健用機能性食品研究会・総会
  • Place of Presentation: 鶴見大学会館（神奈川県・横浜市）
• [Presentation] 糖代謝の生化学的性質からみるBifidobacteriumの齲蝕関連性 (2016)
  • Author(s): 安彦 友希, 菅原 敦信, 村上 和弘, 川嶋 順子, 高橋 信博
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2016-08-24
• [Presentation] 齲蝕関連Bifidobacteriumの酸産生活性とそのフッ化物による抑制効果 (2016)
  • Author(s): 馬目 歩実, 安彦 友希, 川嶋 順子, 福本 敏, 高橋 信博
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌コンベンションセンター（北海道・札幌市）
  • Year and Date: 2016-08-24
• [Presentation] Inhibitory effects of fluoride on the carbohydrate metabolism of oral Bifidobacterium (2016)
  • Author(s): Ayumi Manome, Yuki Abiko, Junko Kawashima, Satoshi Fukumoto, Nobuhiro Takahashi
  • Organizer: Innovative Research for Biosis-Abiosis Itelligent Interface Symposium
  • Place of Presentation: Gonryo Kaikan(Sendai, Japan)
  • Year and Date: 2016-01-18
  • Int'l Joint Research
• [Presentation] Nitrogenous compounds stimulate the growth and acid-production of oral Actinomyces (2014)
  • Author(s): Junko Kawashima, Yuka Norimatu, Teruko Yamamoto-Takano, Hidetoshi Shimauchi, Akito Tsuboi and Nobuhiro Takahashi
  • Organizer: The 62th Annual Meeting of Japanese Association for Dental Research
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2014-12-04 – 2014-12-05
• [Presentation] Stimulatory effects of nitrogenous compounds on the acid production of oral Streptococcus and Actinomyces (2014)
  • Author(s): Yuka Norimatu, Junko Kawashima, Teruko Takano-Yamamoto, Nobuhiro Takahashi
  • Organizer: 第5回 インターフェイス口腔健康科学国際シンポジウム
  • Place of Presentation: 東北大学 片平キャンパス 片平さくらホール
• [Presentation] 口腔Actinomycesの酸産生能および増殖能に対する窒素源の効果 (2013)
  • Author(s): 則松 佑佳、川嶋 順子、山本 照子、高橋 信博
  • Organizer: 第55回 歯科基礎医学会学術大会・総会
  • Place of Presentation: 岡山コンベンションセンター