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Creation of root caries model for evaluation and development of antibacterial biomaterials

Research Project

Project/Area Number 25861784
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Conservative dentistry
Research InstitutionTohoku University

Principal Investigator

Mayanagi Gen  東北大学, 歯学研究科, 助教 (10451600)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsISFET微小pH電極 / pH / エナメル質 / 象牙質 / 根面う蝕 / う蝕関連細菌 / 脱灰 / インターフェイス / ISFET微小pH電極 / pH
Outline of Final Research Achievements

The mineral solubility of coronal enamel and root dentin were evaluated using a model of tooth/bacteria interface. The pH changes at the interface were measured by a miniature ion-sensitive field-effect transistor pH electrode and the amounts of calcium dissolved from tooth surfaces were measured by a fluorescent dye. As a result, it was suggested that root dentin is more soluble to the bacteria-induced acidification than coronal enamel, and that the minerals dissolved from tooth surfaces could raise pH at the tooth/bacteria interface. The method employed in the present study could be useful for evaluating the caries-preventive effects on tooth surface treatment.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (29 results)

All 2017 2016 2015 2014 2013

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 1 results,  Open Access: 1 results) Presentation (24 results) (of which Int'l Joint Research: 6 results)

  • [Journal Article] pH Response and Tooth Surface Solubility at the Tooth/Bacteria Interface2017

    • Author(s)
      Mayanagi G, Igarashi K, Washio J, Takahashi N
    • Journal Title

      Caries Res

      Volume: 51 Issue: 2 Pages: 160-166

    • DOI

      10.1159/000454781

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] <b>PCR-dipstick DNA chromatography for profiling of a subgroup of cariesassociated bacterial species in plaque from healthy coronal surfaces and periodontal </b><b>pockets </b>2016

    • Author(s)
      Tian L, Sato T, Niwa K, Kawase M, Mayanagi G, Washio J, Takahashi N
    • Journal Title

      Biomedical Research

      Volume: 37 Issue: 1 Pages: 29-36

    • DOI

      10.2220/biomedres.37.29

    • NAID

      130005128375

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microbiologically induced corrosive properties of the titanium surface2014

    • Author(s)
      Fukushima A, Mayanagi G, Nakajo K, Sasaki K, Takahashi N
    • Journal Title

      J Dent Res

      Volume: 93(5) Issue: 5 Pages: 525-529

    • DOI

      10.1177/0022034514524782

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of fluoride-releasing restorative materials on bacteria-induced pH fall at the bacteria -material interface : An in vitro model study2014

    • Author(s)
      Mayanagi G, Igarashi K, Washio J, Domon-Tawaraya H, Takahashi N
    • Journal Title

      J Dent

      Volume: 42(1) Issue: 1 Pages: 15-20

    • DOI

      10.1016/j.jdent.2013.11.006

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fluoride-sensitivity of growth and acid production of oral Actinomyces : comparison with oral Streptococcus2013

    • Author(s)
      Kawashima J, Nakajo K, Washio J, Mayanagi G, Shimauchi H, Takahashi N
    • Journal Title

      Microbiol Immunol

      Volume: 57(12) Issue: 12 Pages: 797-804

    • DOI

      10.1111/1348-0421.12098

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Presentation] ペットボトルの口の部分に付着する細菌の量および構成(A pilot study)2017

    • Author(s)
      曾田彩花、佐藤拓一、石黒和子、安彦友希、真柳 弦、鷲尾純平、高橋信博
    • Organizer
      第6回口腔保健用機能性食品研究会・総会
    • Place of Presentation
      鶴見大学会館(神奈川県)
    • Related Report
      2016 Annual Research Report
  • [Presentation] スマートISFETを用いた口腔微小環境マルチイオン測定2017

    • Author(s)
      真柳弦、五十嵐公英、鷲尾純平、中條和子、土門ひと美、高橋信博
    • Organizer
      東北大歯学研究科-東工大未来研学術連携シンポジウム
    • Place of Presentation
      東北大学大学院歯学研究科(宮城県)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 歯質-細菌インターフェイスにおける歯質脱灰の評価2017

    • Author(s)
      真柳弦、高橋信博、益一哉
    • Organizer
      平成28年度生体医歯工学共同研究拠点成果報告会
    • Place of Presentation
      東京医科歯科大学(東京都)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Evaluation of pH using an ISFET at the bacteria/restorative materials interface2016

    • Author(s)
      Mayanagi G, Igarashi K, Washio J, Nakajo K, Domon H, Takahashi N
    • Organizer
      International Symposium on Biomedical Engineering
    • Place of Presentation
      東京医科歯科大学(東京都)
    • Year and Date
      2016-11-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Corrosive effects of fluoride on titanium under artificial biofilm of Streptococcus mutans2016

    • Author(s)
      Fukushima A, Mayanagi G, Sasaki K, Takahashi N
    • Organizer
      The 11th International Workshop on Biomaterials in Interface Science  Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2016
    • Place of Presentation
      東北大学大学院歯学研究科(宮城県)
    • Year and Date
      2016-08-30
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of fluoride on titanium discoloration and elution under biofilm2016

    • Author(s)
      Fukushima A, Mayanagi G, Sasaki K, Takahashi N
    • Organizer
      45th Annual meeting & Exhibition of the American Association for Dental Research
    • Place of Presentation
      Los Angeles, USA
    • Year and Date
      2016-03-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Fluoride-coated tooth surface inhibits bacteria-induced pH fall at the biofilm/tooth interface2016

    • Author(s)
      Ishiguro T, Mayanagi G, Fukushima A, Sasaki K, Takahashi N
    • Organizer
      Innovative Research for Biosis-Abiosis Intelligent Interface Symposium -The 6th International Symposium for Interface Oral Health Science-
    • Place of Presentation
      東北大学艮陵会館(宮城県)
    • Year and Date
      2016-01-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] PCR-dipstick DNA chromatography for multiplex and semi-quantitative analysis of plaque biofilm microbiota2016

    • Author(s)
      Tian L, Sato T, Niwa K, Kawase M, Mayanagi G, Abiko Y, Washio J, Takahashi N
    • Organizer
      Innovative Research for Biosis-Abiosis Intelligent Interface Symposium -The 6th International Symposium for Interface Oral Health Science-
    • Place of Presentation
      東北大学艮陵会館(宮城県)
    • Year and Date
      2016-01-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ISFETのう蝕研究への展開2016

    • Author(s)
      真柳弦、五十嵐公英、鷲尾純平、中條和子、土門ひと美、高橋信博
    • Organizer
      第5回生体医歯工学公開セミナー
    • Place of Presentation
      東京工業大学すずかけ台キャンパス(神奈川県)
    • Related Report
      2016 Annual Research Report
  • [Presentation] S-PRGフィラー含有コンポジットレジンのStreptococcus mutansの糖代謝活性に対する抑制効果2015

    • Author(s)
      三木彩希、真柳弦、安彦友希、騎馬和歌子、北川晴朗、北川蘭奈、林美加子、高橋信博、今里聡
    • Organizer
      日本歯科保存学会2015年度秋季学術大会
    • Place of Presentation
      文京シビックホール(東京都)
    • Year and Date
      2015-11-12
    • Related Report
      2015 Research-status Report
  • [Presentation] Effect of fluoride on titanium corrosion under Streptococcus mutans biofilm2015

    • Author(s)
      Fukushima A, Mayanagi G, Sasaki K, Takahashi N
    • Organizer
      The 63rd annual meeting of Japanese Association for Dental Research
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2015-10-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 人工バイオフィルム下におけるチタンの微生物腐食性2015

    • Author(s)
      福島梓、真柳弦、中條和子、佐々木啓一、高橋信博
    • Organizer
      粉体粉末冶金協会平成27年度春季大会
    • Place of Presentation
      早稲田大学国際会議場(東京都)
    • Year and Date
      2015-05-26
    • Related Report
      2015 Research-status Report
  • [Presentation] PCR-dipstick DNA Chromatography for Semi-quantitative Analysis of Oral Microbiota2015

    • Author(s)
      Tian L, Sato T, Niwa K, Mayanagi G, Kawase M, Tanner A.C.R, Takahashi N
    • Organizer
      93rd General Session & Exhibition of the International Association for Dental Research
    • Place of Presentation
      Boston, USA
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Fluoride-coating on Tooth Surface Inhibits Bacteria-induced pH-fall at Biofilm-tooth Interface2015

    • Author(s)
      Ishiguro T, Mayanagi G, Fukushima A, Sasaki K, Takahashi N
    • Organizer
      93rd General Session & Exhibition of the International Association for Dental Research
    • Place of Presentation
      Boston, USA
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Effects of bacterial oxygen consumption on corrosive properties of the titanium surface2014

    • Author(s)
      Fukushima A, Mayanagi G, Nakajo K, Sasaki K, Takahashi N
    • Organizer
      NIH-Japan-JSPS Symposium 2014
    • Place of Presentation
      Maryland, USA
    • Year and Date
      2014-10-23 – 2014-10-24
    • Related Report
      2014 Research-status Report
  • [Presentation] バイオフィルム-歯面インターフェイスpH測定装置を用いた各種フッ化物のpH低下抑制効果の評価2014

    • Author(s)
      石黒朋子、真柳弦、佐々木啓一、高橋信博
    • Organizer
      第56回歯科基礎医学会学術大会
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2014-09-26 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] PCR-dipstick DNA chromatography for multiplex analysis of oral microbiota2014

    • Author(s)
      Tian L, Sato T, Niwa K, Mayanagi G, Yamaki K, Kawase M, Tanner A.C.R, Takahashi N
    • Organizer
      第56回歯科基礎医学会学術大会・サテライトシンポジウム
    • Place of Presentation
      福岡国際会議場(福岡県)
    • Year and Date
      2014-09-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Microbiologically induced corrosive properties of the titanium surface under artificial biofilm of Streptococcus mutans2014

    • Author(s)
      Fukushima A, Mayanagi G, Nakajo K, Sasaki K, Takahashi N
    • Organizer
      9th International Workshop on Biomaterials in Interface Science (Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2014)
    • Place of Presentation
      宮城蔵王ロイヤルホテル(宮城県)
    • Year and Date
      2014-08-26 – 2014-08-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Glucose-induced acidification and tooth surface solubility at tooth-bacteria interface2014

    • Author(s)
      Mayanagi G, Igarashi K, Washio J, Takahashi N
    • Organizer
      Innovative Research for Biosis-Abiosis Intelligent Interface Symposium -The 5th International Symposium for Interface Oral Health Science-
    • Place of Presentation
      東北大学片平さくらホール(宮城県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Microbiologically-induced corrosive property of titanium under artificial biofilm of Streptococcus mutans2014

    • Author(s)
      Fukushima A, Mayanagi G, Nakajo K, Sasaki K, Takahashi N
    • Organizer
      Innovative Research for Biosis-Abiosis Intelligent Interface Symposium -The 5th International Symposium for Interface Oral Health Science-
    • Place of Presentation
      東北大学片平さくらホール(宮城県
    • Related Report
      2013 Research-status Report
  • [Presentation] Involvement of Tooth Surface Solubility in Stephan Curve2013

    • Author(s)
      Mayanagi G, Igarashi K, Washio J, Takahashi N
    • Organizer
      2nd Meeting of the International Association for Dental Research -Asia Pacific Region
    • Place of Presentation
      Bangkok, Thailand
    • Related Report
      2013 Research-status Report
  • [Presentation] Involvement of oxygen-concentration in microbiologically-induced corrosion of titanium under biofilm2013

    • Author(s)
      Fukushima A, Mayanagi G, Nakajo K, Sasaki K, Takahashi N
    • Organizer
      2nd Meeting of the International Association for Dental Research -Asia Pacific Region
    • Place of Presentation
      Bangkok, Thailand
    • Related Report
      2013 Research-status Report
  • [Presentation] pH response and mineral solubility of tooth root surface at tooth-bacteria interface2013

    • Author(s)
      Mayanagi G, Igarashi K, Washio J, Takahashi N
    • Organizer
      8th International Workshop on Biomaterials in Interface Science -Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2013-
    • Place of Presentation
      さんさ亭(宮城県)
    • Related Report
      2013 Research-status Report
  • [Presentation] Possible involvement of oxygen concentration cell in microbiologically-induced titanium corrosion2013

    • Author(s)
      Fukushima A, Mayanagi G, Nakajo K, Sasaki K, Takahashi N
    • Organizer
      International College of Prosthodontists
    • Place of Presentation
      Turin, Italy
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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