Induction of odonto-/osteoblastic differentiation by FGF18 and its clinical application

• Project/Area Number: 25861787
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Ohi Chie 東京医科歯科大学, 歯学部, 非常勤講師 (30431935)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: FGF18 / FGFシグナル / 硬組織分化 / 歯髄幹細胞 / 骨芽細胞 / 分化誘導 / Akt / 細胞増殖 / 歯髄細胞 / 細胞分化 / 象牙芽細胞 / Wnt / FGF2 / 分化 / 硬組織形成 / 歯髄 / 石灰化

Outline of Final Research Achievements

Fibroblast growth factors (FGF)18 is a famous growth factor involved in development/differentiation of bone/cartilage tissues. This project focused on the effects of FGF18 on pulpal and osteoblastic differentiation. FGF18 promoted the cell growth of human dental pulp stem cells, mouse calvaria-derived osteoblasts, and mouse osteoblast-like cells: MC3T3-E1. It also enhanced alkaline phosphatase and DSPP mRNA expression. These results indicate that FGF18 possesses an ability to induce differentiation/maturation of hard-tissue forming cells. Clinical application of FGF18 may effectively induce differentiation/mineralization of odonotoblasts and osteoblasts.

Research Products

• [Presentation] 歯髄組織および歯肉組織より得られた間葉系幹細胞の硬組織形成細胞への分化能の比較 (2015)
  • Author(s): 川島伸之、山本弥生子、橋本健太郎、Alumddin Bakhit、小泉悠、辺見浩一、大井智恵、鈴木規元、興地隆史
  • Organizer: 日本歯科保存学会春季大会
  • Place of Presentation: 北九州国際会議場（北九州・小倉）
  • Year and Date: 2015-06-25
• [Presentation] ALPase Activity of Pulp Cells on EDTA and NaClO-treated Dentin (2015)
  • Author(s): K. Hashimoto, N. Kawashima, A. Y. Bakhit, M. Yamamoto, Y. Koizumi, S. Takahashi, C. Ohi, N. Suzuki
  • Organizer: IADR/AADR/CADR General Session
  • Place of Presentation: Hynes Convention Center, Boston, MA、USA
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] Mineralizing Properties of Gingiva-derived Mesenchymal Stem Cells Cultured in 3D-condition (2015)
  • Author(s): N. Kawashima, K. Hashimoto, A. Y. Bakhit, M. Yamamoto, Y. Koizumi, S. Takahashi, C. Ohi, N. Suzuki
  • Organizer: IADR/AADR/CADR General Session
  • Place of Presentation: Hynes Convention Center, Boston, MA, USA
  • Year and Date: 2015-03-11 – 2015-03-14