Neuron-immune Interactions in the Sensitized Thalamus Induced by Pulpitis

• Project/Area Number: 25861793
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KAWAMURA Jun 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (20634083)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: グリア / ニューロン / 歯痛 / グリア細胞 / 痛み / 歯髄炎 / 神経障害性疼痛 / 歯髄 / ミクログリア / アストロサイト

Outline of Final Research Achievements

We have recently reported that the signal of pulp injury induces both neuronal and glial cell activation in the contralateral thalamus in rats, although the
mechanisms of the glial cell/neuronal interaction remain unclear. This study was undertaken to test our hypothesis that p38 mitogen-activated protein kinase (MAPK) signaling pathways are involved in the pulp injury-induced glial cell/neuronal interaction in the thalamus.The area immunopositive to phospho-p38 MAPK increased until 72 hours after pulp exposure in both local anesthetic-pretreated and saline-pretreated animals. The density of glial fibrillary acidic protein-expressing astrocytes showed a significant increase only in the saline-pretreated animals. Expression levels of MAPK 13 and MAPK 14 mRNAs increased at 24 hours and still higher at 72 hours in the saline-pretreated animals.

Research Products

• [Journal Article] Up-regulation of p38 mitogen-activated protein kinase during pulp injury-induced glial cell/neuronal interaction in the rat thalamus. (2013)
  • Author(s): Kaneko T, Chokechanachaisakul U, Kawamura J, Yamanaka Y, Ito T, Sunakawa M, Suda H, Okiji T.
  • Journal Title: J Endod. Volume: 39 Issue: 4 Pages: 488-92
  • DOI: 10.1016/j.joen.2012.11.018
  • Peer Reviewed
• [Presentation] 実験的歯髄炎はラット視床におけるグリア細胞を活性化させる (2015)
  • Author(s): 河村 隼、金子友厚、砂川光宏、興地隆史
  • Organizer: 日本歯科保存学会
  • Place of Presentation: 文京シビックホール（東京都文京区）
  • Year and Date: 2015-11-12
• [Presentation] Local anesthetic pretreatment suppresses activation of astrocytes as antigen presenting cells in the rat thalamus following dental pulp inflammation (2014)
  • Author(s): Jun Kawamura, Tomoatsu Kaneko, Yusuke Yamanaka, Takafumi Ito, Mitsuhiro Sunakawa, Takashi Okiji, Hideaki Suda
  • Organizer: 13th INTERNATIONAL SYMPOSIUM ON DENDRITIC CELLS
  • Place of Presentation: Tours(France)
  • Year and Date: 2014-09-14 – 2014-09-18
• [Presentation] 実験的歯髄炎はラット視床におけるミクログリアを活性化させる (2013)
  • Author(s): 河村 隼，金子友厚，山中裕介、伊藤崇史、砂川光宏、興地隆史、須田英明
  • Organizer: 第138回日本歯科保存学会2013年度春季学術大会
  • Place of Presentation: 福岡国際会議場
• [Presentation] Glial Cell/neuronal Interaction in Thalamus following Dental Pulp Inflammation (2013)
  • Author(s): Jun Kawamura, Tomoatsu Kaneko, UraiwanChokechanachaisakul, Yusuke Yamanaka, Takafumi Ito, Mitsuhiro Sunakawa, Takashi Okiji, Hideaki Suda
  • Organizer: 第2 回IADR アジア太平洋地区歯科研究学会（2nd IADR-APR Meeting）
  • Place of Presentation: タイ・バンコク