| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
This study attempted to analyze the mRNA and protein expression of Mrp4, Pgt and EPs receptors with real-time PCR and immunofluorescent staining in lipopolysaccharide-inflamed rat incisor pulp tissue. Moreover, the amounts of PGE2 released from the inflamed pulp tissue in the presence of absence of inhibitors were assessed by using an enzyme-linked immunosorbent assay. Mrp4, Pgt, and mPGES expression were detected in the endothelial cells of normal and LPS-inflamed rat incisor pulp tissue, suggesting that these cells are associated with the biosynthesis and transmembrane efflux transport pathway of PGE2. Moreover, EP2 and EP4 were expressed in odontoblasts and endothelial cells, suggesting that EP2 and EP4 might play various roles in dental pulp inflammation by binding the PGE2.
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