Membrane transport protein mediatted prostaglandin E2 transport in experimentally inflmaed rat dental pulp
| Project/Area Number |
25861794
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Conservative dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
OHKURA Naoto 新潟大学, 医歯学総合病院, 医員 (00547573)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | トランスポーター / プロスタグランジンE2 / MRP4 / PGT / EPレセプター / EP2 / EP4 / MRP４ |
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| Outline of Final Research Achievements |
This study attempted to analyze the mRNA and protein expression of Mrp4, Pgt and EPs receptors with real-time PCR and immunofluorescent staining in lipopolysaccharide-inflamed rat incisor pulp tissue. Moreover, the amounts of PGE2 released from the inflamed pulp tissue in the presence of absence of inhibitors were assessed by using an enzyme-linked immunosorbent assay. Mrp4, Pgt, and mPGES expression were detected in the endothelial cells of normal and LPS-inflamed rat incisor pulp tissue, suggesting that these cells are associated with the biosynthesis and transmembrane efflux transport pathway of PGE2. Moreover, EP2 and EP4 were expressed in odontoblasts and endothelial cells, suggesting that EP2 and EP4 might play various roles in dental pulp inflammation by binding the PGE2.
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Report
(4 results)
Research Products
(17 results)
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[Presentation] う蝕治療のトピックス2014
Author(s)
大倉直人
Organizer
第２回分子薬剤学セミナー
Place of Presentation
富山大学薬学部 (富山県富山市)
Year and Date
2014-11-15
Related Report
Invited
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