| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Outline of Final Research Achievements |
This study aimed to examine the involvement of angiogenetic factors in the development of experimental apical periodontitis in rat molars. Induction of apical periodontitis under the administration of an NF-kB inhibitor (NF-kB decoy) resulted in the reduction of (1) periapical radiolucent area, (2) CD31-positive endothelial cell density, and (3) mRNA expression levels of VEGFR2, CXCL1 and CXCR2.When rat endothelial cells and mesenchymal stem cells were co-cultured in the presence of LPS, addition of NF-kB decoy resulted in the downregulation of CXCL1 and CXCR2 mRNA expression in these cells and a significant decrease of VEGF in the culture medium. These results suggest that NF-kB signaling pathways in endothelial cells play a role in the pathogenesis of apical periodontitis.
|
