| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
The precise periapical healing mechanism induced by root canal treatment (RCT) is unknown. We reported previously that interleukin (IL)-1a, but not IL-1b, and laminin were expressed during the healing phase in an experimental rat RCT model. However, the mechanism of the interaction between IL-1a and laminin for periapical healing is not clear. In this study, we investigated the biological effects of IL-1a and laminin on mouse osteoblastic cells, MC3T3-E1, and rat RCT model. IL-1a (0.1 ng/ml) and laminin (10 ng/ml) enhanced the phosphorylation of FAK and MAPKs (ERK1/2, p38, JNK1/2) in MC3T3-E1. In addition, IL-1a and laminin enhanced the mRNA expression of type1 collagen. We tried to visualize the inflammation of periapical region using molecular imaging. Inflammation of periapical region in rat RCT model was detected and quantified using molecular imaging. These data suggest that the dual effects of IL-1a and laminin may enhance the induction of osteogenesis during periapical healing.
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