| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
We have previously reported that the inflammatory cytokine, tumor necrosis factor α(TNF α) inhibited BMP-induced osteoblast differentiation via NF-κB signaling. In the present study, we examined effects of steroid-coactivator, MTI-II, on inflammatory responses in osteoblasts. When the cells were treated with MTI-II, TNFα-induced NF-κB-luciferase activity failed to increase in a dose-dependent manner, as well as TNFα-induced expression of MMP-9 and IL-6 mRNA, a target gene of NF-κB. We next examined effects of MTI-II on the inhibition of BMP-induced osteoblastogenesis by TNFα. When the cells were treated with TNFα, BMP-induced ALP activity was inhibited, while MTI-II restored TNFα-mediated inhibition of the activity in a dose-dependent manner. These results suggest that MTI-II involves in the inhibition of BMP-induced osteoblastogenesis by TNFα, and that MTI-II may have an potential as an anti-inflammatory drug.
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