The expression and function of midkine in human periapical granulomas

• Project/Area Number: 25861812
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Nihon University
• Principal Investigator: HATORI Keisuke 日本大学, 歯学部, 助教 (30632583)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: midkine / CXCL12 / CXCL13 / CX3CL1 / 血管内皮細胞 / 歯根肉芽腫 / 根尖性歯周炎

Outline of Final Research Achievements

Two-color immunofluorescense image analysis indicate that co-expression of midkine(MK) and chemokine(CXCL12, CXCL13 or CX3CL1) in human periapical granulomas. Furthermore, MK and chemokines messenger RNA expression in periapical granulomas was significantly higher than healthy gingival tissues by real time PCR analysis. In addition, the MK and CX3CL1 mRNA expression in stimulated human vascular endothelial cells was significantly higher than negative control. On the other hand, CXCL12 mRNA expression was significantly lower than negative control.
These data indicated the possibility that MK, CXCL12, CXCL13 and CX3CL1 related to pathogenesis and growth of human periapical granulomas.

Research Products

• [Journal Article] Epstein-barr virus infection in chronically inflamed periapical granulomas. (2015)
  • Author(s): Makino K, Takeichi O, Hatori K, Imai K, Ochiai K, Ogiso B
  • Journal Title: PLos One Volume: 10 Issue: 4 Pages: e0121548-e0121548
  • DOI: 10.1371/journal.pone.0121548
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of trigeminal transition zone and laminated subnucleus caudalis in masseter muscle hypersensitivity associated with tooth inflammation. (2014)
  • Author(s): Shimizu K, Matsumoto K, Noma N, Matsuura S, Ohara K, Komiya H, Watase T, Ogiso B, Tsuboi Y, Shinoda M, Hatori K, Nakaya Y, Iwata K.
  • Journal Title: PLoS OＮＥ Volume: 9(10) Issue: 10 Pages: e109168-e109168
  • DOI: 10.1371/journal.pone.0109168
  • Peer Reviewed / Open Access
• [Presentation] Co-expression of Midkine and Chemokine in Human Periapical Granulomas (2015)
  • Author(s): K Hatori, O Takeichi, K Makino, K Ishii, K Komiyama, M Maeno, B Ogiso
  • Organizer: 第93回国際歯科学会
  • Place of Presentation: ボストン(米国)
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] Inflammatory and Anti-inflammatory Reactions in Periapical Granulomas (2015)
  • Author(s): O Takeichi, K Makino, K Hatori,H Kudo, B Ogiso
  • Organizer: 第93回国際歯科学会
  • Place of Presentation: ボストン(米国)
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] The Evidence of the Epstein-barr Virus Infection in Periapical Granulomas (2015)
  • Author(s): K Makino, O Takeichi, K Hatori, H Kudo, K Imai, K Ochiai, B Ogiso
  • Organizer: 第93回国際歯科学会
  • Place of Presentation: ボストン(米国)
  • Year and Date: 2015-03-11 – 2015-03-14
• [Presentation] 歯根肉芽腫中の血管内皮細胞におけるmidkineおよびchemokine発現 (2013)
  • Author(s): 羽鳥啓介、武市 収、牧野公亮、小木曾文内
  • Organizer: 第６回日本口腔検査学会総会・学術大会
  • Place of Presentation: 鶴見大学会館(神奈川県)
• [Presentation] 難治性根尖性歯周炎におけるmidkineとiNOS発現 (2013)
  • Author(s): 武市 収、牧野公亮、羽鳥啓介、小木曾文内
  • Organizer: 第６回日本口腔検査学会総会・学術大会
  • Place of Presentation: 鶴見大学会館(神奈川県)
• [Presentation] 歯根肉芽腫におけるEpstein-Barr virusの検出 (2013)
  • Author(s): 牧野公亮、武市 収、羽鳥啓介、勝呂 尚、今井健一、落合邦康、小木曾文内
  • Organizer: 第139回日本歯科保存学会2013年度秋季学術大会
  • Place of Presentation: 秋田県総合生活文化会館(秋田県)
• [Presentation] Midkine and iNOS expression by endothelial cells in periapical granulomas (2013)
  • Author(s): O.Takeichi、 K.Makino、K.Hatori、M.Maeno、B.Ogiso
  • Organizer: 46th Meeting of the Continental European Division of International Association for Dental Research
  • Place of Presentation: Firenze Fira(Firenze、Italia)