Analysis of heat denaturation layer of an Er:YAG laser irradiated-dentin and development of new adhesive for lased-dentin

• Project/Area Number: 25861815
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: KATO Chikage 日本歯科大学, 新潟生命歯学部, 助教 (00459926)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2014: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 象牙質 / Er:YAG laser / 接着 / コンポジットレジン / Er：YAG laser

Outline of Final Research Achievements

Following experimental conditions, cavity preparation and surface conditioning of the cavity, were conducted using Er:YAG laser; ExpG1: #120~#600 waterproof abrasive paper (Control), ExpG2: 50mJ/10pps irradiation, ExpG3: 150mJ/10pps irradiation, ExpG4: 250mj/10pps irradiation, ExpG5: 50mJ/10pps →150mJ/10pps irradiation, ExpG6: 150mJ/10pps irradiation→ H3PO4(30sec), ExpG7:150mJ/10pps irradiation→ H3PO4(30sec)→ NaClO(90sec), ExpG8: 150mJ/10pps irradiation→ H3PO4(30sec)→ silane coupling treatment, ExpG9: 150mJ/10pps→ 50mJ/10pps irradiation.
Azan Stains were employed heat denaturation layers colored in red were observed in the laser irradiation groups. Little changes were observed on SEM but large changes were observed on specimens in laser irradiation groups of TEM. The collagen fibers structure changes were led by the above mentioned same factors. On EPMA observation, concentration declines of both Ca, P, and Mg were observed some groups.

Research Products

• [Presentation] Er:YAG laser照射面の熱変性層について (2014)
  • Author(s): 加藤千景
  • Organizer: 日本歯科保存学会2014年度秋季学術大会（第141回）
  • Place of Presentation: 山形テルサ
  • Year and Date: 2014-10-30 – 2014-10-31