periodontal tissue engineering by cultured cell sheet, fibroblast growth factor 2 and beta-tricalcium phosphate collagen composite scaffold

• Project/Area Number: 25861879
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Hokkaido University
• Principal Investigator: TAKEFU Hiroe 北海道大学, 大学病院, 助教 (40609103)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 歯周組織再生 / β-TCPコラーゲンスキャホールド / FGF2 / 培養細胞シート / β-TCPコラーゲンスキャフォールド

Outline of Final Research Achievements

The purpose of the present study was determine: (1)the novel method of cultured cell sheet using bone marrow cell from F344/Jcl rats’ femur; (2)the　effectiveness of the cultured bone marrow cell sheet, β-TCP collagen composite scaffold and FGF-2 on bone regeneration of rats’ calvariae.
The results were as follows: (1)the multilayered osteoblast-like cell sheet was produced by this novel method. The proper culture term made this cell sheet more rigid; (2)the complex of　β-TCP collagen composite scaffold and FGF-2 made large amount of new bone on rats’ calvariae.