The analysis of the mechanism of neuritogenesis by pulsed electromagnetic field and the control method of the elongating neurite direction
Project/Area Number |
25861880
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Dental engineering/Regenerative dentistry
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Research Institution | Tohoku University |
Principal Investigator |
Tada-aki Kudo 東北大学, 歯学研究科(研究院), 助教 (50431606)
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Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | パルス磁場刺激 / PC12細胞 / 神経細胞分化 / 温熱効果 / 励磁コイル |
Outline of Final Research Achievements |
Previously, we developed a novel method for inducing neuritogenesis (NG) using a pulsed electromagnetic field (PEMF) with an exciting coil in rat-derived PC12 cells, which are a model of neuronal differentiation. However, the mechanism that enhances NG is still unknown. In the present study, we examined the mechanism of NG due to PEMF, the effect of Joule heating on NG, and the possibility of PEMF affecting the direction of NG. We demonstrated that the bone morphogenetic protein (BMP) pathway is essential in the process of PEMF-induced NG and that the Joule heat generated by the coil partially contributes to the PEMF-mediated induction of neuronal differentiation. We also found that it is possible to efficiently differentiate the cells into neuronal cells by thermal stimulation alone and that it is difficult to regulate the direction of NG using a mono-directional and parallel magnetic field with a custom-made exciting coil as used in the present study.
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Report
(4 results)
Research Products
(8 results)
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[Journal Article] Induction of neurite outgrowth in PC12 cells treated with temperatur-contrilled repeated thermal stimulation.2015
Author(s)
Tada-aki Kudo, Hiroyasu Kanetaka, Kentaro Mochizuki, Kanako Tominami, Shoko Nunome, Genji Abe, Hiroyuki Kosukegawa, Toshihiko Abe, Hitoshi Mori, Kazumi Mori, Toshiyuki Takagi, Shin-ichi Izumi
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Journal Title
PLOS ONE
Volume: 10
Issue: 4
Pages: e0124024-e0124024
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Induction of neurite outgrowth in PC12 cells treated with temperature-controlled repeated thermal stimulation.2015
Author(s)
Tada-aki Kudo, Hiroyasu Kanetaka, Kentaro Mochizuki, Kanako Tominami, Shoko Nunome, Genji Abe, Hiroyuki Kosukegawa, Toshihiko Abe, Hitoshi Mori, Kazumi Mori, Toshiyuki Takagi, Shin-ichi Izumi
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Journal Title
PLOS ONE
Volume: in press
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Investigation of hyperthermic effect on neuronal differentiatino and cell growth in PC12 cells2014
Author(s)
Tada-aki Kudo, Hiroyasu Kanetaka, Kentaro Mochizuki, Kanako Tominami, Toshihiko Abe, Hitoshi Mori, Kazumi Mori, Genji Abe, Hiroyuki Kosukegwa, Toshiyuki Takagi, Shin-ichi Izumi
Organizer
The 5th International Symposium for Interface Oral Health Science
Place of Presentation
仙台
Related Report
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[Presentation] Stimulation of neuritogenesis in PC12 cells by a pulsed electromagnetic field via MEK-ERK1/2 signaling2014
Author(s)
Hajime Takada, Tada-aki Kudo, Hiroyasu Kanetaka, Toshihiko Abe, Hitoshi Mori, Kazumi Mori, Genji Abe, Hiroyuki Kosukegawa, Toshiyuki Takagi, Shin-ichi Izumi, Masahiko Kikuchi
Organizer
The 5th International Symposium for Interface Oral Health Science
Place of Presentation
仙台
Related Report
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