| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Previously, we developed a novel method for inducing neuritogenesis (NG) using a pulsed electromagnetic field (PEMF) with an exciting coil in rat-derived PC12 cells, which are a model of neuronal differentiation. However, the mechanism that enhances NG is still unknown. In the present study, we examined the mechanism of NG due to PEMF, the effect of Joule heating on NG, and the possibility of PEMF affecting the direction of NG. We demonstrated that the bone morphogenetic protein (BMP) pathway is essential in the process of PEMF-induced NG and that the Joule heat generated by the coil partially contributes to the PEMF-mediated induction of neuronal differentiation. We also found that it is possible to efficiently differentiate the cells into neuronal cells by thermal stimulation alone and that it is difficult to regulate the direction of NG using a mono-directional and parallel magnetic field with a custom-made exciting coil as used in the present study.
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