| Project/Area Number |
25861894
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Health Sciences University of Hokkaido |
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Principal Investigator |
YUI tomoo 北海道医療大学, 歯学部, 助教 (80548438)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2013: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
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| Keywords | 破骨前駆細胞 / ニコチン / 破骨細胞 / smooth基質 / Groove基質 / micro-box / TRAP染色 / SEM / Pyk2 / Micro-Gap / RAW264.7 / RANKL / M-CSF |
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| Outline of Final Research Achievements |
Osteoclast precursor cells( RAW264.7 ) were cultured on the three types of grooved substrata with the presence of nicotine. Observation with a stereoscopic microscope confirmed that the cells were positively stained with Tartrate Resistant Acid Phosphatase (TRAP) on the substrata, indicating that they were osteoclasts. Scanning Electron Microscopy (SEM) observation revealed that the osteoclasts were polygonal or round in shape, arranged in a disorganized pattern on the smooth substratum. These results suggest that presence of nicotine was unaffected. In addition , these findings indicate that the gene expression of Pyk2 was unaffected by the presence of nicotine concentration.
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