Basic research for periodontal tissue regeneration type implant using periodontal ligament cells

• Project/Area Number: 25861901
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Tsurumi University
• Principal Investigator: YAMAMOTO Ryuji 鶴見大学, 歯学部, 助教 (20410053)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯周組織再生 / 歯根膜 / 象牙質 / 歯槽骨 / セメント質 / インプラント / 象牙芽細胞 / 歯髄 / アパタイトコーティング

Outline of Final Research Achievements

The purpose of this research was verifying the possibility of the periodontal ligament regeneration around the tooth implant.
In this study I revealed the following. (1) The ceria-stabilized tetragonal zirconia polycrystals/ aluminium oxide nanocomposite (Ce-TZP) has a same ability for bone induction compared to titanium. (2) In transplantation experiments of titanium implants to type 2 diabetes model rat. Nerve growth factor (NGF) was effective for bone induction to surrounding the implant. (3) The different of the two splice variants of porcine dentin sialophosphoprotein (DSPP) gene were revealed between dental pulp cells and odontoblasts. From this result, it showed that DSPP gene is useful as a differentiation marker of odontoblasts.

Research Products

• [Journal Article] Dentin Sialophosphoprotein-derived Proteins in the Dental Pulp. (2015)
  • Author(s): Yamamoto R, Oida S, Yamakoshi Y.
  • Journal Title: Journal of dental research Volume: 印刷中 Issue: 8 Pages: 1120-1127
  • DOI: 10.1177/0022034515585715
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Differentiation potential of osteoblast from cultured C2C12 cells on zirconia disk (2014)
  • Author(s): Saito M1, Karakida T, Yamamoto R, Nagano T, Yamakoshi Y, Hayakawa T, Oida S, Gomi K.
  • Journal Title: Dental Materials Journal Volume: 33 Issue: 2 Pages: 275-283
  • DOI: 10.4012/dmj.2013-321
  • NAID: 10029495211
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed
• [Presentation] DSPP-derived Proteins are Necessary for Maintaining TGF-β1 (2015)
  • Author(s): Ryuji Yamamoto, Saeko Kinoshita, Yasuo Yamakoshi
  • Organizer: 93RD GENERAL SESSION & EXHIBITION OF THE IADR
  • Place of Presentation: Boston(USA)
  • Year and Date: 2015-03-11 – 2015-03-15
• [Presentation] ブタ歯髄から分離した細胞の不死化とクローニング (2014)
  • Author(s): 唐木田丈夫、山本竜司、大井田新一郎、山越康雄
  • Organizer: 第56回 歯科基礎医学会学術大会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] ブタ象牙質シアロリンタンパク質（DSPP）遺伝子のスプライスバリアントについて (2014)
  • Author(s): 山本竜司、唐木田丈夫、大井田新一郎、山越康雄
  • Organizer: 第56回 歯科基礎医学会学術大会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2014-09-25 – 2014-09-27