QCM nano analysis of the adsorption behavior of pellicle protein for the development of infection control material

• Project/Area Number: 25861902
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Tsurumi University
• Principal Investigator: YOSHIDA Eiji 鶴見大学, 歯学部, 助教 (30410054)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: QCM / AFM / 吸着 / バイオフィルム / 歯科材料 / タンパク吸着

Outline of Final Research Achievements

The aim of the present study was to evaluate the adsorption behaviors of saliva pellicle proteins onto a gold, silica and titanium by using the quartz crystal microbalance method. As pellicle proteins, lactoferrin, lysozyme, defensin and mucin were evaluated. Adsorption amount of lactoferrin to silica was significantly lower than gold and titanium. Significant differences were detected between titanium and silica for the adsorption amounts of lysozyme. Chemical bond formation of sulfur atom of lysozyme and gold could be suggested. There were no significant differences of the adsorption amount of β-defensin among each substrate. For mucin adsorption, gold showed the highest adsorption amount. It is presumed that electrostatic repulsion caused less adsorption amounts of mucin to titanium and silica. In conclusion, the differences of the adsorption behaviors of pellicle proteins could be clearly identified.

Research Products

• [Journal Article] 分子プレカーサ-法にて作製したアパタイト薄膜の原子間力顕微鏡観察 (2014)
  • Author(s): 吉田英史 早川徹
  • Journal Title: 日本歯科産業学会誌 Volume: 28 Pages: 9-16
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Biofilm Bormation on Titanium and Hydroxyapatite Surface using Artificial Mouth System (2013)
  • Author(s): Eiji Yoshida, Susumu Imai, Nobuhiro Hanada, Tohru Hayakawa
  • Journal Title: Journal of Hard Tissue Biology Volume: 22 Issue: 4 Pages: 419-424
  • DOI: 10.2485/jhtb.22.419
  • NAID: 130004480611
  • ISSN: 1341-7649, 1880-828X
  • Peer Reviewed
• [Journal Article] Adsorption study of pellicle proteins to gold, silica and titanium by quartz crystal microbalance method (2013)
  • Author(s): Eiji Yoshida, Tohru Hayakawa
  • Journal Title: Dental Materials Journal Volume: 32 Issue: 6 Pages: 883-887
  • DOI: 10.4012/dmj.2013-136
  • NAID: 10031203544
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed
• [Presentation] ラクトフェリンの吸着特性 (2014)
  • Author(s): 吉田英史 早川徹
  • Organizer: 第36回バイオマテリアル学会
  • Place of Presentation: タワーホール船堀（東京都江戸川区）
  • Year and Date: 2014-11-17 – 2014-11-18
• [Presentation] QCM法によるラクトフェリンの吸着特性解析 (2014)
  • Author(s): 吉田英史 早川徹
  • Organizer: 第64回日本歯科理工学会
  • Place of Presentation: アステールプラザ（広島県広島市）
  • Year and Date: 2014-10-04 – 2014-10-05