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Study of genome copy number alterations in oral cancer by FNA-FISH

Research Project

Project/Area Number 25861913
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Surgical dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Myo Kunihiro  東京医科歯科大学, 歯学部附属病院, 医員 (50634119)

Project Period (FY) 2013-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords遺伝子解析 / 口腔扁平上皮癌 / FNA-FISH / SNPマイクロアレイ / ゲノムコピー数変異 / CNA / FNA-FISH法 / コピー数変異 / FISH / FNA
Outline of Final Research Achievements

In oral tongue cancer, lymph node metastasis is poor-prognostic factor. To understand the mechanisms underlying metastasis, we compared the genetic profiles of paired primary tumor (PT) and metastatic lymph node (MLN) samples in oral tongue cancer patients.
We extracted genomic DNA from formalin-fixed and paraffin-embedded (FFPE) tissue sections of paired PT and MLN samples. We applied the past experience of molecular genetic analysis by FNA-FISH, large-scale genetic profiling was performed using high-density single-nucleotide polymorphism (SNP) microarray. Then, we compared the genetic profiles of PT and MLN samples to identify specific copy number alterations (CNA). As a result, we identified 20q11.2 gain was significantly more frequent in MLN samples. Moreover, the expression of one of 20q11.2 genes, E2F1, was significantly increased during process of metastasis. This study indicates that additional genetic changes may be required for the metastatic process.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2016 2015 2013

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Characterizing Genetic Transitions of Copy Number Alterations and Allelic Imbalances in Oral Tongue Carcinoma Metastasis2016

    • Author(s)
      Morita T, Uzawa N, Mogushi K, Sumino J, Michikawa C, Takahashi KI, Myo K, Izumo T, Harada K
    • Journal Title

      Genes Chromosomes Cancer

      Volume: 55 Issue: 12 Pages: 975-986

    • DOI

      10.1002/gcc.22395

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] 緊急対応を要した下顎骨動静脈奇形の1例2013

    • Author(s)
      名生邦彦、長谷川和樹、宮本日出雄、原田清
    • Journal Title

      日本口腔外科学会誌

      Volume: 59(5) Pages: 319-320

    • NAID

      130004714387

    • Related Report
      2013 Research-status Report
  • [Presentation] 舌癌同一患者における原発巣-転移リンパ節間のゲノム異常の相同性と相違性について2015

    • Author(s)
      森田琢磨、鵜澤成一、茂櫛薫、名生邦彦、高橋謙一郎、道川千絵子、炭野淳、出雲俊之、原田清
    • Organizer
      第53回日本癌治療学会総会・学術集会
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2015-10-29
    • Related Report
      2015 Research-status Report
  • [Presentation] Genome-wide comparison of copy number alterations between primary and metastatic site in oral cancer.2015

    • Author(s)
      Takuma Morita, Narikazu Uzawa, Kaoru Mogushi, Kunihiro Myo, Ken-Ichiro Takahashi, Chieko Michikawa, Jun Sumino, Yujiro Moriya, Toshiyuki Izumo, Kiyoshi Harada.
    • Organizer
      第39回日本頭頸部癌学会学術集会;第4回アジア頭頸部癌学会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2015-06-04
    • Related Report
      2015 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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