Identification of osteoclastic bone resorption regulatory factors produced by oral squamous cell carcinoma
| Project/Area Number |
25861916
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 口腔癌 / 骨破壊 / 骨吸収 |
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| Outline of Final Research Achievements |
1. Two clonal cell lines, HSC3-C13 and HSC3-C17, were isolated from the maternal oral cancer cell line, HSC3. The conditioned medium from HSC3-C13 cells showed the highest induction of Rankl expression in the mouse stromal cell lines ST2 and UAMS-32 as compared to that in maternal HSC3 cells and HSC3-C17 cells. Gene expression was compared between HSC3-C13 and HSC3-C17 cells by using microarray analysis, which showed that CXCL2 gene was highly expressed in HSC3-C13 cells as compared to HSC3-C17 cells. These results indicate that CXCL2 is involved in bone destruction induced by oral cancer.
2. Transforming growth factor beta (TGF-b) plays a significant role in the regulation of the tumor microenvironment. We investigated the role of TGF-b in oral cancer-induced bone destruction.We show that both cancer cells and stromal cells produce TGF-b in the OSCC-induced bone destruction, and propose the role of TGF-b produced by stromal fibroblasts in such bone destruction.
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Report
(4 results)
Research Products
(2 results)
