Functional screening of tumor suppressive microRNA and application to nucleic acid medicine

• Project/Area Number: 25861918
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Uesugi Atsushi 東京医科歯科大学, 歯学部附属病院, 医員 (20637061)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 口腔がん / microRNA / 核酸医薬

Outline of Final Research Achievements

This study aims for identification of the tumor suppressive microRNA which is available for clinical application as new nucleic acid medicine contributing to cancer personalized medicine.
We transfected synthetic double strand miRNA library into NA,SKN3 oral squamous cell carcinoma cell lines and RT7 immortalization cell line derived from an oral mucosa epithelium .As a result, we recognized cytostasis activity in a cancer cell lines and picked miRNA not to accept on RT7.We perform analysis about the identification of target molecules and the molecular mechanism.