| Project/Area Number |
25861928
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Mie University |
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Principal Investigator |
OKUMURA Kenya 三重大学, 医学(系)研究科(研究院), 助教 (50582079)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 遺伝子治療 / 口腔がん / Bax mRNA / Liposome / mRNA / 口腔悪性腫瘍 / Bax |
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| Outline of Final Research Achievements |
Gene transfer by mRNA lipofection is more efficient than plasmid DNA lipofection. We study the gene therapy using a new method, Bax mRNA lipofection for oral malignant tumors (Bax is a pro-apoptotic molecule that functions as a tumor suppressor.). In this study, we examined the antitumor effect in nude mice transplanted tumors of the Bax mRNA transfected using a cationic liposome against human mandibular osteosarcoma (HOSM-1 cells) and human squamous cell carcinoma (KB cells), and studied its antitumor effects comparing the differences between local administration and systemic administration.
In both mandibular osteosarcoma and human squamous cell carcinoma, Bax mRNA therapy using liposomes has more anti-tumor effects than Bax gene therapy using a plasmid, and intravenous administration was the most effective route of administration in this study.
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