Analysis of TGF-beta/Smad signal pathway involved in metastasis of oral squamous cell carcinoma
| Project/Area Number |
25861934
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 口腔扁平上皮癌 / 頸部リンパ節転移 / TGF-β / 運動能 / EMT / TGF-beta / リンパ節転移 |
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| Outline of Final Research Achievements |
We exaimned the effect of TGF-β on the highly lymph nodes metastatic oral squamous cell carcinoma cell line, LM8 and its parental cell, SAS-GFP. We found that TGF-β didn't mediate their morphological change, cell to cell contact and growth. But, TGF-β induced increase in their migration and invasiveness. Epithelial-mesenchymal transition was inclined to be induced through up regulation of N-cadherin and Vimentin and enlogation of actin fiber in TGF-β stimulated cells. Additionaly, the expression of Wnt5b was elevated, not Wnt5a. Over all, these results suggest that TGF-β could increase migration and invasion of highly lymph nodes metastatic oral squamous cell carcinoma cell through EMT, in which Wnt5b could be involved.
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Report
(5 results)
Research Products
(7 results)
