Differentiation potency analysis of maxillofacial genetic disease derived iPS cells by serum-free embryoid culture

Research Project

Project/Area Number	25861945
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Surgical dentistry
Research Institution	Hiroshima University
Principal Investigator	NABESHIMA Kou 広島大学, 大学病院, 歯科診療医 (10550999)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	iPS細胞 / 再生医療 / iPS
Outline of Final Research Achievements	Healthy subject and cleidocranial dysplasia derived iPS cells was generated the cell mass called the embryoid body using activin A. This embryoid body was cultured for a long term of 30 days and analyzed it about the induced organization.Tissue section prepared cultured embryoid body, which has examined histologically, not showed clear cartilaginous tissue. However, tissue section staining and genetic analysis suggested that induced embryoid body have been cartilaginous tissue of maxillofacial region.