| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We assessed the growth inhibitory effect of LNA ASO for miR-361-3p in vivo using a mouse model. But there was no significant difference compared to the control group. It did not also observed side effects such as weight loss and loss of appetite in mice. LNA ASO was transfected into primary cultured cells at the concentration of 10 nM. As in the case of OSCC cell lines, knockdown of miR-361-3p induced the growth inhibition of OSCC primary cultured cells.
Furthermore, bioinformatic and microarray analyses indicated that miR-361-3p and miR-133ab could target OSR2 mRNA and CEBPA mRNA.
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