| Project/Area Number |
25861955
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
Ryoji Kitamura 九州大学, 歯学研究院, 共同研究員 (60637338)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | サイトケラチン１７ / サイトケラチン１９ / サイトケラチン２０ / 口腔扁平上皮癌 / 予後因子 / 末梢血単核球 / real-time PCR / 末梢血 / 腫瘍マーカー / サイトケラチン17 / サイトケラチン１３ |
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| Outline of Final Research Achievements |
Cytokeratins (CKs) are intermediate filaments of the cytoskeleton and candidate prognostic biomarkers for OSCC, as they are over-expressed in OSCC compared with normal mucosa. The aim of this study was to determine the relative levels of CK17, CK19 and CK20 mRNAs in peripheral blood mononuclear cells (PBMC) using real-time RT-PCR. The study comprised pre- and post-operative PBMC samples from 20 OSCC patients, from which, CK17, CK19 and CK20 mRNA was examined by real-time RT-PCR. In the good-prognosis group, 10 of 13 patients showed reduced CK17 mRNA expression in their post-operative compared with pre-operative samples, conversely, only three of six patients in the poor-prognosis group had reduced post-operative CK17 expression. This difference was statistically significant (p<0.01). In conclusion, CK17 expression may be a useful prognostic biomarker for OSCC.
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